UltiMate 3000 Series: Flow Managers and Column Thermostats
Page
82
Operating Instructions
Long term interruptions of over a week:
To shut down the flow manager:
1.
Flush the column(s) according to manufacturer recommendations.
2.
Prime all lines with an eluent containing more than
40% isopropanol
.
3.
Stop the pump flow.
4.
In the Chromeleon
Commands
dialog box (F8), select
ColumnOven ->
FlowSplitter_1
and set
FSControlMode = Off
.
5.
Run the pump at a 1000 µL/min Master flow for 5 minutes to flush the FCV.
6.
Stop the pump and switch the system off.
To start up the flow manager:
1.
Set the
FSControlMode = Off
before
starting the pump.
2.
Flush the system thoroughly at 1000
µ
L/min master flow for 5 minutes.
3.
Stop the pump flow.
4.
Set
FSControlMode = Auto
to restore normal operation of the system.
5.
To check if the FCV achieves sufficient restriction for typical nano LC conditions
before reconnecting the column, set
FSColPressDesired
= 150 bar and
FSControlMode = Pressure
with a master flow of 300
µ
L/min.
Summary of Contents for UltiMate FLM-3100
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