Applied Biosystems GeneAmp PCR System 9700, User Manual

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5-14 Creating and Editing Methods
Deleting Holds
Follow the procedure below to delete holds.
Inserting Cycles
Follow the procedure below to insert cycles.
Inserting
Programmed Pauses
Follow the procedure below to insert a programmed pause.
To delete holds:
1 From the
Edit
screen, select a hold parameter and press
More
.
2 Use the Circular Key Pad to a select a time or temperature hold parameter.
3 Press
Delete
.
2 Pre-PCR 3 Tmp 25 Cycles 2 Holds
55.0
94.0
2:00
10:00
55.0
0:30
72.0
0:30
72.0
5:00
4.0
94.0
0:30
*
F1 F3 F4 F5 F2
Delete Insert More
To insert cycles:
Step Action
1 From the
Create
screen, use the Circular Key Pad to select a time or temperature
parameter to the right of where you want to insert a cycle.
2 Press
More
.
Depending on the parameter you select in step 1, one of three screens displays
from which you can access the insert function.
3 Press Insert . The Insert screen appears.
4 Press
Cycle
to insert a cycle to the left of the segment you selected in step 2.
Note You can delete a cycle by entering 0 in the
Tmp
field.
2 Pre-PCR 3 Tmp 25 Cycles 2 Holds
55.0
94.0
2:00
10:00
55.0
0:30
72.0
0:30
72.0
5:00
4.0
94.0
0:30
*
F1 F3 F4 F5 F2
Hold Cancel Pause Cycle
To insert a programmed pause:
Step Action
1 From the Create screen, use the Circular Key Pad to select a PCR segment time or
temperature parameter where you want to insert a programmed pause.
2 Press
More
.
3 Press
Insert
. The Insert screen appears.