Applied Biosystems GeneAmp PCR System 9700, User Manual

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Creating and Editing Methods 5-13
Inserting Holds
Use the Insert function to insert holds and cycles into your method, and program
pauses that the instrument automatically inserts into your method as it runs.
5 Modify the up-ramp and down-ramp rates you selected by defining them as a
percentage of the maximum of 100%:
Note You can also use the numeric keys to enter a value that defines the
percentage by which you want to decrease the ramp rate for each cycle of the
method. You can enter values between 5 and 95, or 100.
For ramp rates less than 100%, an asterisk * appears next to modified ramps. The
asterisk remains beneath the modified temperature parameter to remind you that
the method has been modified.
Note The following message appears if you enter a numeric value outside the
range of acceptable values, “Valid range is 5 to 95 and 100.”
6 Press
Accept
to accept all entries, and return to the previous screen.
To modify ramp rates:
(continued)
Step Action
If you want to... Then press...
increase the ramp rate by 10% up to a maximum of 100% the
Faster
key.
decrease the ramp rate by 10% from 100% to 10%, and by 5%
from 10% to 5% the
Slower
key.
To insert holds:
Step Action
1 From the
Create
screen, use the Circular Key Pad to select a time or temperature
parameter to the right of where you want to insert a hold.
2 Press
More
.
Depending on the parameter you select in step 1, one of three screens displays
from which you can access the insert function.
3 Press
Insert
. The Insert screen appears.
Note The Pause function will not display on the Insert screen if the highlighted
segment already has a pre-programmed pause or if highlight box is not on a PCR
segment parameter.
4 Press Hold to insert a hold of 4.0 °C for 30 seconds to the left of the parameter you
selected in step 1.
5 Type in a value for the hold temperature.
6 Type in a value for the hold time.
7 Press More to return to the Create screen. Your modified method now appears.
2 Pre-PCR 3 Tmp 25 Cycles 2 Holds
55.0
94.0
2:00
10:00
55.0
0:30
72.0
0:30
72.0
5:00
4.0
94.0
0:30
*
F1 F3 F4 F5 F2
Hold Cancel Pause Cycle