Applied Biosystems GeneAmp PCR System 9700, User Manual

Page: 58 / 132

4-2 Running PCR Samples
Selecting a Method
Introduction
After you have prepared your samples and loaded them in the sample block, you can
run a PCR amplification with a new or a stored method.
What Is a Method
A method is a set of instructions in which you specify how the instrument should heat
and cool your samples in a PCR thermal profile.
Methods are stored in the instrument software.
Predefined Methods
The GeneAmp
®
PCR System 9700 supplies eight predefined methods that you can
run:
♦
AmpliCycle ® Sequencing
♦
AmpliTaq Gold
®
♦
BigDye™
♦
General PCR
♦
LSM2
♦
Time Release PCR
♦
Touchdown PCR
♦
XL PCR
Each of these methods is stored under the user name <<pe>>. You can edit these
methods and store them under a different name, a different user name, or select any
one and run it.
For a detailed description of each of these pre-coded methods, and how you can use
them, see Appendix B, “Supplied Methods.”