Applied Biosystems GeneAmp PCR System 9700, User Manual

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5-10 Creating and Editing Methods
Defining Post-PCR
Holds
On the Create screen, the Holds field defines the number of temperature control
parameters in the post-PCR segment of your method.
The post-PCR incubation temperature and hold time parameters define how to hold
your samples at a specified temperature until you are ready to analyze them.
Note If the idle state setpoint, or the last hold of the Method are below 15 °C, then the heated
cover will automatically set to 50 °C.
Post-PCR Parameter Settings
Typical Post-PCR parameter settings:
Follow the procedure below to define Post-PCR Holds
4 Create the first hold-time parameter:
a. Press Enter .
b. Type in a hold-time value between 00:00 and 98:59 (minutes:seconds).
5 Repeat step 3 and step 4 until you have time and temperature values for each of the
segment temperature control parameters you defined in step 1.
To define the PCR parameters:
(continued)
Step Action
Temperature Time (min:sec) Use For
72 °C 7:00 Complete extension of all amplicons
72 °C 99:59 (×) AmpErase
™
applications
4 °C 99:59 (×) General storage
To define post-PCR holds:
Step Action
1 Select the
Holds
field.
2 In the Holds field, type in the number of post-PCR steps for your method.
3 Press
Enter
to select the first post-PCR temperature parameter.
4 Type in a temperature value between 4.0 °C and 99.9 °C.
5 Press
Enter
to select the first post-PCR hold time parameter.
6 Type in a hold time value between 00:00 and 98:59 (min:sec).
Note The hold time × indicates a hold that lasts indefinitely. You can enter an ×
hold time, by typing a hold time value of 99:00 or greater.
7 Press Enter . This selects the next temperature parameter.
8 Repeat step 4 through step 7 until you have time and temperature values for each
of the post-PCR hold parameters you defined in step 2.