Applied Biosystems GeneAmp PCR System 9700, User Manual

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5-8 Creating and Editing Methods
Entering
Temperature
Control Parameters
When you enter temperature control parameters, you define values for parameters in
each of the three segments of a method: pre-PCR, PCR, and post-PCR.
Figure 5-1 Order of advancement of the highlight box
To enter temperature control parameters:
Step Action
1 On the Create screen, select a field.
When you first display the Create screen, the
Hld
field is highlighted.
2 Use the numeric keys to enter values.
3 Press
Enter
to accept a value. The next field is then selected in the order shown in
Figure 5-1.
Number Description
1 Number of pre- PCR holds
2 Pre-PCR temperature parameter
3 Pre-PCR hold time parameter
4 Number of PCR segment temperatures
5 Number of cycles for the PCR segment
6 PCR segment temperature parameter
7 PCR segment time parameter
8 PCR segment temperature parameter
9 PCR segment time parameter
10 PCR segment temperature parameter
11 PCR segment time parameter
12 Number of post-PCR holds
13 Post-PCR temperature parameter
14 Post-PCR hold time parameter
15 Post-PCR temperature parameter
16 Post-PCR hold time parameter
1 Hld 3 Tmp 25 Cycles 2 Holds
94.0 94.0
5:00 0:30
55.0
0:30
72.0
0:30
72.0
7:00
4.0
F1 F3 F4 F5 F2
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Pre-PCR segment PCR segment Post-PCR segment
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2
3
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12 13
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