Pannoramic 250 FLASH III 3.0.2 User’s Guide
5.1.1 Profile Settings Panel
•
Load scanmap:
This function is useful if the slides contain areas of interest that cannot be
detected by the preview camera. This function along with saving profiles helps to scan all
slides with the most adequate settings.
To load a mask, click
Load
. It will display a drop-down menu. Select whether to load preview
mask or TMA grid.
•
Custom scanmap:
The scanmap draws manually on the preview image is used for scanning.
Use selection box restriction:
If selected, only the area inside the box is scanned. It is useful if
you only want to scan the area that is equivalent with the size of the coverslip, so external
specks will not be scanned.
Multilayer mode
•
Select
Single layer
option if you want to scan a single layer of the slide.
•
By choosing the
Extended focus
option, the software selects the sharpest image from each
focus level for each image field, and combines them into one single image. Therefore those
parts that were blurry in auto/manual focus will also be sharp. This method guarantees the
maximum depth of sharpness, however, it reduces digitization speed.
Figure 29 – Focus level and step size
•
Z-Stack:
For
Extended focus
,
Z-Stack
, and for
Flash Z-Stack
scanning method as well, you can
determine the desired number of focus levels with the
Levels
selection box. The current focus
value is the initial level. If you set
5
for example, then the initial level, plus four (two focus
levels above and below the FOVs) are captured. Therefore, it is advised to choose an odd
number for the focus levels. Separate layers are created and stored this way, and can be
opened with Viewer applications. The Z-Stack slide contains the extended focus layers.
September 16, 2020 – Rev. 1
3DHISTECH Ltd.
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