WPI MICRO-ePUMP, Инструкция по эксплуатации

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MICRO-ePUMP
World Precision Instruments 1
ABOUT THIS MANUAL
The following symbols are used in this guide:
This symbol indicates a CAUTION. Cautions warn against actions that can cause
damage to equipment. Please read these carefully.
This symbol indicates a WARNING. Warnings alert you to actions that can cause
personal injury or pose a physical threat. Please read these carefully.
NOTES and TIPS contain helpful information.
Fig. 1—MIRO-ePUMP Microinjector includes an internal pressurepump and the integrated
MICRO-ePORE Cell Penetrator technology.
INTRODUCTION
Designed to simplify intracellular injection and a variety of other micro in jec tion tasks,
the MICRO-ePUMP uses carefully regulated air pres sure for injecting cells with fluid.
Injected volumes range from picoliters to nanoliters. The port supplies positive pressure
for high-pressure ejection. The pressure port maintains a low positive “compensation”
pres sure to the injecting pipette between injection pulses to prevent fluid up take
through capillary action.
Timing, injection pressure and compensation pressure are adjusted inde pen dent-
ly using the touch screen interface. Time intervals can range from 2 seconds down
to 10 ms or less, depending on the injection pressure setting. The injection pressure
interval is triggered using the foot switch.