WPI MICRO-ePUMP, Инструкция по эксплуатации

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16 World Precision Instruments
Changing the Pipette
To change the pipette, turn off the injection PORT to avoid depleting the reservoir. The
button indicates the present state of the port.
1. To turn off the Injection Port, press the Settings section on the touchscreen.
Fig. 31—The Settings menu lets you save enable or disable the Injection Port by pressing the
Port ON/Port OFF toggle button.
2. Press the Port ON button to disable the Injection Port
3. Press the BACK button to save the settings and return to the main screen. A
warning indicates that the Injection Port is disabled.
Fig. 32—When the Injection Port is disabled a notice appears on the main screen.
4. To install the micropipette, see “Assembling the Capillary Kit” on page 9.
5. When the micropipette is properly installed, click on the Settings area to access
the Settings menu and press the Port OFF button to enable the Injection Port.
TECHNIQUES IN MICROINJECTION
The MICRO-ePUMP was designed for demanding tasks like the microinjection of fluid
into cells. In this section, we will look at several important things to keep in mind when
working with the
MICRO-ePUMP
.
Setting the Compensation Pressure
The compensation pressure is used to counterbalance the capillary action of the fluid
backfilling into the pipettes. If you insert an empty pipette into fluid, you can see a
meniscus rising from the capillary tip.
Set the Compensation Pressure parameter by touching the Compensation Pressure
parameter on the main screen and adjusting the knob until the meniscus stops at the
desired position in the micropipette. In many applications, colored dye or fluorescent