WPI MICRO-ePUMP, Инструкция по эксплуатации

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2 World Precision Instruments
The MICRO-ePUMP is designed to inject very small quantities of fluids, such as drugs,
into cells or small organelles. Pressure injection is an especially useful alternative to
electroionophoresis, since it does not mandate the use of charged ions. Two different
positive pressures may be applied–one for ejection at high pressure and a second,
lower pressure to prevent back filling of the pipette by capillary action.
WPI’s MICRO-ePORE™ Pinpoint Cell Penetrator technology is embedded inside the
MICRO-ePUMP . When the researcher enables the The MICRO-ePORE™ , it delivers
a highly localized voltage signal to a targeted injection site to facilitate penetration
with minimal trauma. The researcher determines the amplitude and frequency of the
signal that best suits the application. The signal originates in the control box, and it
is transmitted through the electrode interface cable to the microelectrode holder.
A silver wire is used to transmit the signal into the electrically conductive substance
being injected. A reference electrode is used to place the media at 0.0 V potential with
reference to the generated voltage.
The
MICRO-ePUMP offers separate regulated compensation (back filling prevention)
and ejection pres sures with a pre ci sion timing circuit that switches from injection
pressure to compensation pressure au to mat i cal ly.
Features
• Regulated compensation and injection pressure
• Pressure output: 0.3-87 PSI
• Onboard MICRO-ePORE™
• Pinpoint Cell Penetrator for targeted microinjection and increased viability of
injected embryos
• Audio continuity tone indicating active probe
• Injection counter to indicate total number of injections
• User-adjustable frequency and voltage through touch screen
• Foot switch to activate voltage for MICRO-ePORE™ and injection
• Low volume tubing assembly
Benefits
• Internal pressure source
• Intuitive user-interface for injector and MICRO-ePORE™
• Small footprint takes up very little bench space
• Easy to navigate with touch screen and control knob
• Inject into a single cell in picoliter volumes
Applications
• Microinjection of diverse compounds and biomolecules – DNA, RNA, proteins
• Pre- and post-implantation in embryos of various species – mice, rats, monkeys,
bovine, pigs, zebrafish, etc.