Woodley InSight V5, Руководство по обслуживанию

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2 Instrument Configuration
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reaches the measurement section of the flow chamber. The sampling syringe, at a constant
speed and within a certain period of time, pushes the sample fluid wrapped in the sheath flow
so that it travels steadily through the flow chamber for optical measurement.
➢
WBC parameters measurement
After the first section sheath flow is measured, add the quantitative V5 LY1 Lyse into WBC
reaction bath to make it react fully. After that, place the sample between the inlet of the flow
chamber and sampling syringe, and open the sheath flow and make it stable, then the sheath
flow wrapping the sample reaches the measurement section of the flow chamber. The
sampling syringe, at a constant speed and within a certain period of time, pushes the sample
fluid wrapped in the sheath flow so that it travels steadily through the flow chamber for optical
measurement.
The measurement channels are cleaned after measurement to restore them to their
pre-measurement status.
2.2.3.2 Measurement Process
The measurement process for the DIFF measurement module is as follows:
1. Dosing: The diluent syringe is first applied to add the diluent into the WBC bath, and then the
sample probe is used to add the blood sample into the WBC bath, where they are mixed with V5
LY2 evenly for incubation.
2. Mixing: Open Valve LV19 to generate air bubbles through the intermittent valve opening to mix
the sample well.
3. Measurement: Aspirate the incubated sample from the WBC bath using the sampling syringe,
then close LV12, and open LV01 and LV05, so that the sheath flow syringe can push the sheath
fluid into the flow chamber to form a stable sheath flow. Then push the sample into the flow
chamber via the sample injection tube. This is how the sample, wrapped in the sheath flow,
enters the measurement section for measurement.
The measurement process for the WBC measurement module is as follows:
1. Dosing: The diluent syringe is first applied to add the diluent into the WBC bath, and then the
sample probe is used to add the blood sample into the WBC bath, where they are mixed evenly.
After aspirating the diluted sample for the RBC channel measurement, V5 LY1 Lyse is added into
the WBC bath for mixing and incubation.
2. Mixing: Open Valve LV19 to generate air bubbles through the intermittent valve opening to mix
the sample well.
3. Measurement: Aspirate the incubated sample from the WBC bath using the sampling syringe,
then close LV12, and open LV01 and LV05, so that the sheath flow syringe can push the sheath
fluid into the flow chamber to form a stable sheath flow. Then push the sample into the flow
chamber via the sample injection tube. This is how the sample, wrapped in the sheath flow,
enters the measurement section for measurement.
4. Cleaning: To clean, open LV01 and LV07, and add the diluent into the WBC bath using the
dosing syringe.
5. Waste discharge: Waste is discharged by opening Valve LV14, LV15 and Pump P1.