Turner Designs TD-700, Руководство по эксплуатации

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TD-700 Laboratory Fluorometer Operating Manual
Page 53
APPENDIX 12
Instructions for Using the Solid Standard with the
TD-700 Fluorometer
1. Calibrate your instrument with a known standard as you usually do per
sections VII - IX.
2. Remove calibration standard and any cuvette adaptors from instrument.
3. Facing the instrument, place the solid standard in the filter cylinder with the
letter “L” (etched on top of standard holder) on your left hand side.
Standards are marked with the letter “L” to designate low concentration and
“H” to designate high concentration.
4. Press <*> to read value. Record this number on the label located at the
bottom of the standard as the low concentration, “L” .
5. Now pull standard out of filter cylinder, rotate standard 180 degrees, and
place in filter cylinder again, so that the letter “H” is on your left hand side.
6. Press <*> to read value. Record this number on the label located at the
bottom of the standard as the high concentration, “H".
7. Remove solid standard and replace any cuvette adaptors that were removed
in step 2.
8. Record instrument settings on TD-700 Calibration Record with Solid
Standard sheet that has been included for future reference.
9. Proceed with analyzing your samples.
10. You may use your solid standard at any time to check the stability of the
instrument. Simply remove any cuvette adaptors and insert your standard.
Press <*> to read. The value should be similar to what was previously
obtained in steps 4 and 6 above.
11. Also, you may use it to calibrate on future days, using the values obtained in
steps 4 and 6 above. For example, if the values obtained for “L” and “H” were
50 and 100 respectively, you can use both to calibrate your instrument on
another day. When calibrating, enter in “100” for
your first standard
concentration “H” and “50” for your second concentration “L”: If calibrating
with only one of the concentrations, enter in the corresponding value (50 or
100) depending on whether “L” or “H” is on your left side when facing the
instrument.