Turner Designs TD-700, Руководство по эксплуатации, Страница: 26 / 55

TD-700 Laboratory Fluorometer Operating Manual
Page 25
6. Fill a clean test tube or cuvette with the Hi Std (highest concentration
standard you are using). Wipe the outside of the cuvette dry and insert
it into the sample adaptor in the sample chamber. Press <*>. The unit
will adjust sensitivity (as shown by the SENS FACTOR), to the level
appropriate for that standard then read the standard.
7. If you are using only 1 standard, the TD-700 will prompt you to insert
the Blank. If you are using 2 or more standards, the unit will prompt
you to enter the actual concentration of the second standard (#2 Std,
as in step 5) and to insert the second standard (#2 Std, as in step 6),
then the third (#3 Std), etc. Use a clean, rinsed test tube or cuvette
and insert the next standard; press <*>; then <ENT> when finished.
8. When all the standards have been run, the TD-700 will prompt you to
insert the Blank. Fill a clean test tube or cuvette with the Blank, wipe
the outside dry, and insert it into the sample adaptor in the sample
chamber. Press <ENT>.
9. Wait for the Blank reading to stabilize, then press <0>. The TD-700
will read the Blank, then automatically return to the HOME screen.
Calibration data will printout automatically if connected to a printer or
computer. (See Appendix 2 for details regarding the calibration data
printout.)
B. How Sample Concentrations will be Calculated
As you have read, the Direct Concentration calibration procedure accepts
multiple standards and one blank. The concentration vs. fluorescence plot
of both the standard and the blank will result in something close to a
straight line (assuming the standards are within the linear range of the
assay and instrument). If you draw a straight line from point to point on the
calibration plot and record the slope of the line between each point, you
can use these slope values to calculate to sample concentrations. For
example, the graph below displays a plot of five standards and a blank.
Drawing a line between each point will give you five different linear
segments. If the fluorescence of a sample reads between point 2 and point
3, the slope of the segment that connects point 2 and point 3 (segment 3)
is used to calculate the direct concentration of this sample.
The following equation would be used to calculate sample concentration
0
50
100
150
200
250
300
350
400
0 100 200 300 400 500 600
Actual Concentration of Standards Measured
Segment 3
Point 2
Point 3
Insert Hi Std
and press <*>
SETTING sens
Sens Factor: XX
6.
#X Std Conc. XXX.X
1. OK
9. Change
#X Std Conc. XX.X
New: <ENT>
Insert #X Std
and press <*>
Reading: #X
WAIT
7.
Press <0> when
value stable XXX
Insert Blank
and press <ENT>
Reading Blank
WAIT
Blank = XXX
Calibration
Complete
9.
8.
Reading Hi Std
WAIT
Sensitivity Set
Sens Factor: XX