Turner Designs TD-700, Руководство по эксплуатации

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TD-700 Laboratory Fluorometer Operating Manual
Page 52
Excitation Filter: The excitation filter is used to screen out the wavelengths of
light not specific to the compound you are measuring. This filter allows a smaller
band of light energy to pass through and excite the sample solution.
Cuvette: The cuvette or sample cell contains the sample of interest. The cuvette
material must allow the compound's absorption and emission light energy to
pass through (for example, compounds that fluoresce below 300 nm require a
quartz cuvette). The emission light energy will be emitted from the sample
solution at a right angle to the incident light energy. The size of the sample cell
will also effect your measurement. The greater the diameter of the cuvette, the
lower the concentration that can be read.
Emission Filter: Stray light and any background material fluorescence will also
be emitted from the sample solution. The emission filter is chosen to screen out
these components allowing only wavelengths of light specific to the compound to
pass through.
Light Detector: The TD-700 light detector is a photomultiplier tube. The light
allowed to pass through the emission filter, (the wavelengths of light specific to
the compound) are detected by the photomultiplier tube. The light intensity,
which is directly proportional to the compounds' concentration, is then registered
as a digital readout.