ThermoFisher Scientific QuantStudio 6 Pro, Руководство пользователя

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9.
Select an action depending on whether the custom dye calibration passed or
failed.
Calibration
status
Action
Passed
1. Tap Accept Results or Reject Results .
Note:
Accepting the results saves the calibration data to the
instrument and overwrites existing data.
2. (
Optional ) Tap Transfer to EDS to transfer the calibration data
to a USB.
Failed
1. Perform the calibration again using a new custom dye plate.
Create the plate using the next dye concentration greater than
the optimal dye concentration (see “Determine the optimal dye
concentration” on page 100).
2. For more information about calibration failures, see
“Troubleshoot calibration failure” on page 89.
10.
Unload the plate from the instrument.
CAUTION! PHYSICAL INJURY HAZARD. During instrument operation, the
plate temperature can reach 100°C. Allow it to cool to room temperature before
handling.
Note: Use Smart Help if the instrument does not eject the plate (see “Request
technical support with Smart Help” on page 159).
Calibrate for a custom melt curve run
Use this calibration to prepare for high resolution melt analysis runs.
Note:
A custom melt calibration calibrates a custom dye and a melt calibration at the
same time.
• Ensure that all calibrations are current (see “View the calibration status on the
instrument” on page 81).
• Add a custom dye (see “Add a custom dye to the instrument” on page 102).
1.
In the home screen, tap (Settings)
4
Maintenance and
service
4
Calibration
4
Custom melt .
2.
Select the option for the kit that you are using.
•
PCR + Melt
• Melt only
Chapter 7
Calibrate and verify instrument performance
Calibrate for a custom melt curve run
7
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QuantStudio ™ 6 Pro and 7 Pro Real-Time PCR Systems User Guide