ThermoFisher Scientific QuantStudio 6 Pro, Руководство пользователя

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Note: To prepare custom dye plates and to perform custom calibrations, see
“Calibrate custom dyes” on page 98.
Calibration descriptions
The combination of the instrument and the block is calibrated (see “Instrument and
block calibration” on page 79).
Calibration description and purpose Pass criteria
ROI/Uniformity
• The software captures images for each optical filter.
• The software uses calibration data to map the increase in fluorescence
to the plate wells during subsequent runs and to evaluate well
‑
to
‑
well
consistency of the signals.
The image for each filter
distinguishes all wells of the plate.
Each well in the image is distinct.
Background
• The software captures background images for each optical filter in the
absence of sample and reagent, and it checks that the fluorescence
from each well is below a fluorescence threshold.
• The software uses calibration data to remove background fluorescence
during a run.
Note:
You can also run this calibration to determine if contamination is
related to the sample block or the plate.
The plate images for all filters are free
of abnormal fluorescence.
Dye
• The software extracts a spectral profile for each dye standard, then
produces a set of spectral profiles plotted as fluorescence vs filter.
• The software uses calibration data to characterize and distinguish the
individual contribution of each dye in the total fluorescence signals
collected by the instrument.
Dye spectra peak within the same
filter as their group.
View the calibration status and set reminders
View the calibration status on the instrument
The Status and Reminders screen also allows you to perform the following
functions:
• Transfer the calibration results (see page 89)
• Transfer the calibration data files (see page 89)
• View the calibration images (see page 87)
Chapter 7
Calibrate and verify instrument performance
Calibration descriptions
7
QuantStudio ™ 6 Pro and 7 Pro Real-Time PCR Systems User Guide
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