ThermoFisher Scientific QuantStudio 6 Pro, Руководство пользователя

Страница: 93 / 216

1
2 4
5
7
6
3
8
Figure 13 384-well RNase P plate
1
NTC (no template control)
2
STD 1,250 copies
3
STD 2,500 copies
4
STD 5,000 copies
5
STD 10,000 copies
6
STD 10,000 copies
7
Unknown A (5,000)
8
Unknown B (10,000)
Performance specifications pass criteria
After the run, the software calculates average copy number values and standard
deviation values. The instrument passes performance specifications if the following
inequality is true and the instrument successfully distinguishes between unknown
populations A and B with a statistical confidence level of 99.7%.
[(C
qA
) – 3(σ
CqA
)] > [(C
qB
) + 3(σ
CqB
)]
where:
• C
qA
= Average C
q
of unknown population A
• σ
CqA
= Standard deviation of unknown population A
• C
qB
= Average C
q
of unknown population B
• σ
CqB
= Standard deviation of unknown population B
The software automatically adjusts the threshold and omits a defined number of wells
from the unknown populations to meet the performance specifications. To view any
omitted wells, open the data file for the verification in the desktop software.
Prepare an RNase P plate
Materials required for RNase P plate preparation
• RNase P instrument verification plate
• Centrifuge with plate adapter; buckets cleaned before use
• Powder‐free gloves
• Safety glasses
Chapter 7
Calibrate and verify instrument performance
Perform verification using RNase P plates
7
QuantStudio ™ 6 Pro and 7 Pro Real-Time PCR Systems User Guide
93