ThermoFisher Scientific QuantStudio 6 Pro, Руководство пользователя

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3.
Select or add a dye, then select a filter set appropriate for your dye's wavelength
(see filter-wavelength table below).
Note: See your reagent kit documentation for dye name and wavelength
information.
Peak filter
Filter wavelength (nm)
[1]
Excitation Emission
x1-m1 470 ± 15 520 ± 15
x2-m2 520 ± 10 558 ± 12
x3-m3 550 ± 10 587 ± 10
x4-m4 580 ± 10 623 ± 14
x5-m5 640 ± 10 682 ± 14
x6-m6 662 ± 10 711 ± 12
[1]
The central wavelengths are the optimized wavelengths.
IMPORTANT! If the selected filter set does not match your reagent kit
documentation, then the incorrect wavelength may be collected during a run.
4.
(Optional) Tap Reagents , then enter the reagent information.
5.
Load the plate into the instrument
6.
Tap Start .
7.
When the run is complete and the screen displays Calibration complete , tap
View results
4
Details .
8.
Review the plot.
Passing calibration results show uniform signals with peaks that are aligned with
the dye wavelength.
Figure 15 An example dye calibration plot
Note:
The peaks for your dye may align with a different filter set.
Chapter 7
Calibrate and verify instrument performance
Calibrate for a custom melt curve run
7
QuantStudio ™ 6 Pro and 7 Pro Real-Time PCR Systems User Guide
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