Dionex Integral Systems Operator’s Manual
90
Doc. 065250-06 03/2020
6. Using a syringe (P/N 016640) and a luer adapter (P/N 24305), fill the
standard loop with deionized water.
Do not introduce any air into the
loop.
7. Reinstall the plug on the end of the coupler from which the water
exited.
8. Remove the syringe and luer adapter from the other end of the loop
and install the plug in it.
9. Examine the outside of the loop for water droplets.
Carefully
dry any
water and then weigh the loop to the nearest 0.0001gram, if possible.
10. Subtract the weight of the empty loop (
filled loop (
); the difference is the weight of the water in the
standard loop.
11. Repeat
until four to five consecutive weighings
0.009 are achieved.
12. Multiply the weight of the water by 1000 to obtain the standard loop
volume in microliters (
L). The table below lists examples of dilution
factors and final concentrations of the diluted standard.
Standard
Loop Size
(
L)
Dilution
Volume
(mL)
Dilution
Factor
Calibration
Standard
Concentration
(mg/L)
Diluted
Standard
Concentration
(
g/L)
10
50
5000
10
2
20
50
2500
10
4
25
50
2000
10
5
50
50
1000
10
10
100
50
500
10
20
150
50
33
10
30
200
50
250
10
40
250
50
250
10
40
250
25
100
10
100
Содержание Dionex
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