Thermo Scientific Dionex AutoTrace 280, Руководство оператора

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Doc. 065330-05 03/2020
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D • Glossary
Air Factor
The adjustable factor that determines the distance liquid is pushed beyond the 12-
port valve and the switching valves. The air push factor is used in
Solvent
Conditioning and Elute to Soak steps to push the liquid partially through the
cartridge or disk path. This prevents the bed of the cartridge or disk from
becoming dried out.
The selected setting (from 0.3 to 5) increases or decreases, respectively, the
standard 1.2 mL of air that follows the liquid being sent through the cartridge by a
factor of 0.3 to 5 times.
If volumes greater than 5 mL are required, the Dry with Gas step in “Creating a
Method to Clean the Sample Lines” on page 45 is recommended.
Air Push
A flow of air generated by the air push syringe that clears liquid from the liquid
handling syringe and tubing lines after conditioning, rinsing, eluting, and syringe
washing steps.
AutoTrace Software
Software used to set up, create, and review a Dionex AutoTrace 280 method and
then run the method to control operation of the Dionex AutoTrace 280.
Flow Rate
The speed (in mL/min) at which to run the Dionex AutoTrace 280 syringes. Flow
rates for solid-phase conditioning, loading, rinsing, and eluting operations are
designated in the Set Up Parameters window in the AutoTrace software.
Maximum Elution Volume
The maximum volume that can be eluted into the collection container, based on
the container size.
Method
The list of steps that defines the operating conditions for a run. Methods are
created in the AutoTrace software and used by the software to run the samples.