Thermo Scientific Dionex AutoTrace 280, Руководство оператора

Страница: 57 / 166

3 • Operation and Maintenance
Doc. 065330-05 03/2020
49
If... Then...
You are going between immiscible
solvents in the method Perform a Wash Syringe step
with an intermediate solvent
before performing the next step
(even if
Autowash is being
used)
You need syringe washing to appear in
your method printout for auditing
purposes Disable Autowash and use
Wash Syringe method steps
where needed
You are running with the Air Factor
parameter set to less than one
Perform a Cartridge Rinse (or
Disk Rinse ) step between
sample loading and elution
The elution solvent is immiscible in water Perform a Dry with Gas step to
remove all water from the
cartridges or disks and eliminate
channeling
You are not drying the cartridge or disk
after loading sample Perform a Cartridge Rinse (or
Disk Rinse ) step before elution
There is water in your extract Increase the pressure and/or time
of the
Dry with Gas step
The elution solvent is not reaching the
packing material in the first
Elute to
Soak step
Use a volume of 3 mL or greater
in the elution step
You need to increase the contact time
between solvent and packing material for
extraction of analytes Use an Elute to Soak step
before Elute to Collect
You need to minimize the amount of air
introduced between multiple elution steps Use an Elute to Soak step
before Elute to Collect
You need to collect more than the
maximum elution volume of your
collection container Use an Elute to Collect, 2nd
Tube step to double the
collection volume
You need to improve throughput Decrease times wherever
possible
You need to improve recovery See Table 3-1
Table 3-3. Method Step Considerations (Continued)