JuLI™ Stage STAT Software
JuLI™ Stage - User Manual
105
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Note: Finding the best combination of settings is an iterative process and should
always be tested on representative images (different time points, positive and
negative controls, etc.).
Continue comparing results with
Quick Mode
turned off (then Click
Apply
twice). In
Quick mode,
pixels of an image are binned, thereby reducing the processing time
of large data sets. Binning means that neighboring pixels are aggregated to a larger
single pixel representing the mean intensity of the binned pixels.
Quick Mode: on
Quick Mode: off
In this example, the Quick mode improved segmentation, but some background is
still falsely detected in both cases.
Then compare the segmentation result with
Background Control
(automatic
background brightness correction) turned on or off.
Background Control: off
Background Control: on
This greatly improved the separation of background and foreground in this example
image.
Another value to influence the quality of the segmentation is called
Default Gating
.
It automatically gates the intensity graph (manual min/max intensity inputs are
disabled then).
