JuLI™ Stage STAT Software
130
JuLI
TM
Stage - User Manual
Scrolling in the results table to the right will display the result of counted cells per
mm². The size of the imaged position in a well = one Field of View (FOV) depends
on the objective used in the measurement: for
4x the size of one FOV is 3.55 mm²
10x the size of one FOV is 0.58 mm²
20x the size of one FOV is 0.14 mm²
Default settings,
Apply
Applying the default settings to this image results in dim cells not being segmented
and some falsely selected background signals:
Background Control
checked
Using the
Background Control
(effecting an automatic background brightness
correction) results in the false signal being excluded, but still some falsely fused (see
yellow circle for example).