JuLI™ Stage STAT Software
JuLI™ Stage - User Manual
101
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The default setting of the selected measurement always displays the brightfield
image and therefore this channel is presented in light blue. Any other acquired
channel of the measurement is shown in dark blue, not measured channels in grey.
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Note: Only one channel can be selected at a time, no other acquired channel can
be co-selected to create overlays.
Positions in the well are light blue when selected, otherwise are shown in dark
blue.
Using the time lapse slider bar, different time points of a selected well can be
viewed.
Confluence analysis can be calculated on a brightfield image as well as on a
fluorescence image, both having different sliders and parameters to optimize
segmentation results.
Optimizing confluence analysis on brightfield images
There are two parameters on the
BRIGHT Param
tab to influence the segmentation
result of brightfield images:
Sensitivity
and
Background Level
. Under
A
,
B
and
C
different settings for these two parameters are preconfigured.
Depending on the brightness and contrast of the image, they lead to different
segmentation results.
