JuLI™ Stage STAT Software
JuLI™ Stage - User Manual
131
Quick Mode
unchecked
Turning off
Quick mode
improved the cell separation, then falsely fused cell were
separated (yellow circle). But in the example image, some cells were overseparated.
(In
Quick mode,
pixels of an image are binned, thereby reducing the processing
time of large data sets. Binning means that neighboring pixels are aggregated to a
larger single pixel representing the mean intensity of the binned pixels.)
Splitting Factor
changed from 25 to 50
Now, the splitting of cells in the overseparated area improved. The number of
detected cells decreased with increased splitting factor.
In order to exclude cells with low of high intensities from counting, an intensity
gating/thresholding can be applied. A histogram (x-axis: fluorescence intensity, y-
axis: number of objects) as well as a mouse hover information on cells pointed at
with the mouse is available to support the decision process.
