3 Operating Procedures
3 Emission Scan with Emission Autofilter (Scan Figure 3)
When you use Autofilter in Endpoint mode for the emission cutoff filter, a filter is
automatically selected that will block as much of the residual excitation light as possible
without unduly reducing the fluorescence signal.
In Spectrum mode, the default is ‘no filter’. You can determine the optimum filter, as
follows:
a
First select an emission filter that is at least 20 nm larger than the selected excitation
wavelength.
b
Complete emission scan from the filter wavelength over a range of 50 nm to 100 nm
larger.
c
Compare signal to buffer background in the scan and choose the wavelength with the
maximum ration.
d
You may want to repeat the scan with the next larger emission filter and compare
results. An example is shown in the
Scan Figure 3
. Note that the optimal emission
wavelength is 525 nm.
Scan Figure 3
82
FlexStation II Operator’s Manual – Rev. D
Содержание FlexStation II
Страница 6: ...FlexStation II Operator s Manual Rev D vi ...
Страница 92: ...4 Applications 86 FlexStation II Operator s Manual Rev D ...
Страница 102: ...5 Service and Maintenance Procedures 96 FlexStation II Operator s Manual Rev D ...
Страница 124: ...6 Troubleshooting Procedures 118 FlexStation II Operator s Manual Rev D ...
Страница 134: ...7 Appendices 128 FlexStation II Operator s Manual Rev D ...
