Molecular Devices FlexStation II, Руководство оператора

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1.4 System Components
4
The excitation beam is collimated by a mirror to a 1.5mm diameter beam before
entering the sample in the microplate well (in bottom-read mode). This focusing helps
to prevent part of the beam from striking adjacent wells.
5
The light beam enters the well and, if fluorescent molecules are present, light of the
emission wavelength is emitted back out to mirrors that focus it and send it to an optical
bundle.
6
The emission monochromator (also a holographic diffraction grating monochromator)
allows light of the chosen emission wavelength to pass to the emission filter wheel.
7
Long-pass filters in the emission filter wheel further condition the light prior to detection
by the photo-multiplier tube (PMT). Using SOFTmax PRO, you can choose which
filter to use, or use none, or have a filter automatically selected.
8
The PMT detects the emitted light and passes a quantitative signal to the instrument’s
electronics, which then send the data to the computer.
1.4.5 Computer
The FlexStation II instrument works as a system with the SOFTmax PRO application
software. SOFTmax PRO must be installed on a dedicated personal computer to
communicate with and control instrument functions.
•
The instrument is equipped with an 8-pin DIN RS-232 serial port for connecting to
a personal computer.
•
SOFTmax PRO software, version 4.4 or greater, is required to control the
FlexStation II instrument.
•
The minimum computer configuration includes a Pentium processor, with 256K
RAM, a 10 GB hard drive, CD-ROM drive, and Windows 98/2000/NT.
For further information, see sections 2.8, Installing SOFTmax PRO, and 3.4, Setting
Up the Software.
FlexStation II Operator’s Manual – Rev. D 15