3.5 Configuring Instrument Settings in Flex Mode
Transfers
You can enable up to three compound transfers, within a single well, during a run time.
The default value for the number of transfers is one (1). As you enable transfers, color-
coded transfer buttons appear next to the Transfers box.
When you select one of the Transfer buttons, that button will appear with a darker gray
background. You can then enter the parameters for that particular transfer in the
Transfer Settings portion of this dialog box.
Important:
There is nothing else in the Transfer Settings portion of the box to indicate
which transfer you are configuring. Pay close attention to which transfer settings you are
modifying.
Transfer Settings
•
Pipette Height
(1 to 999 µL): This setting determines the volume of fluid (in
microliters, measured from the bottom of the assay plate microplate well) above
which the tip of the pipette will remain during the dispensing portion of the transfer
event. This setting helps ensure that the tip of the pipette is below the surface of the
liquid at the end of the transfer, thus minimizing the possibility that undispensed
drops remain on the tips.
Important!
As you configure subsequent transfers you must calculate the amount of
fluid added and set the pipette height accordingly.
•
Rate
(1–8): This setting determines the rate at which the fluid is dispensed into the
well of the assay microplate. A setting of 1 is equal to 16 µL/second and each
subsequent number increases in increments of 16 microliters. Therefore, a setting of 2
is equal to 32 µL/second, etc. A setting of 3 or 4 may help minimize cell damage or
dislodgment.
Note:
For non-contact dispensing, use a rate of 8 to ensure all liquid is dispensed
from pipette tip.
•
Volume:
This setting determines the volume of material to be dispensed from the
source to each individual well chosen to receive that transfer. For a 96-well
microplate, the range is 1–200 µL. For a 384-well microplate, the range is 1–30 µL.
Important!
Keep in mind the maximum total volume each well can hold as you
accumulate volumes with multiple transfers.
FlexStation II Operator’s Manual – Rev. D
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Содержание FlexStation II
Страница 6: ...FlexStation II Operator s Manual Rev D vi ...
Страница 92: ...4 Applications 86 FlexStation II Operator s Manual Rev D ...
Страница 102: ...5 Service and Maintenance Procedures 96 FlexStation II Operator s Manual Rev D ...
Страница 124: ...6 Troubleshooting Procedures 118 FlexStation II Operator s Manual Rev D ...
Страница 134: ...7 Appendices 128 FlexStation II Operator s Manual Rev D ...
