Molecular Devices FlexStation II, Руководство оператора

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3 Operating Procedures
3.5 Configuring Instrument Settings in Flex Mode
This section explains how to use the Instrument Settings screen (see page 52) to
configure the protocol for your experiments. Protocol parameters for Flex mode are
listed below:
•
3.5.1: Wavelengths
•
3.5.2: Sensitivity
•
3.5.3: Timing
•
3.5.4: Automix
•
3.5.5: AutoCalibrate
•
3.5.6: Assay Plate Type*
•
3.5.7: Wells to Read*
•
3.5.8: Compound Source*
•
3.5.9: Compound Transfer*
•
3.5.10: Triturate Selection
•
3.5.11: Pipette Tips Layout*
•
3.5.12: Compound and Tip Columns*
•
3.5.13: AutoRead
•
3.5.14: Pipette Tip Air Gap
•
3.5.15: Settings Displayed in Plate Sections
*Note: Parameters marked with an asterisk (*) must correlate. See following discussion.
Important! You must define instrument settings before beginning a reading or the
instrument will use default settings. Settings cannot be changed after collecting data.
Modifying Settings
Modify settings in the order in which they appear in the Instrument Settings screen to
avoid missing any settings. However, the Timing setting depends on several settings
which appear after it in the list. You will need to return to Timing after completing all
settings to obtain the minimum read interval.
It is essential that settings for the fluidics operations are correct and correlate with one
another. In particular, layouts and settings for Assay Plate Type, Wells to Read,
Compound Source, Compound Transfer, Pipette Tips Layout, and Compound & Tip
Columns must correlate. (These settings are marked with an asterix (*) in the list above.)
SOFTmax PRO software will cross-check these settings as you move through the
configuration windows and when you click OK to close the Instrument Settings screen.
If settings do not correlate, an error message will appear and you must correct settings
before continuing.
Finishing Settings
After you configure all Flex instrument settings to your specifications, click [OK] at the
bottom of the window to return to the Experiment Window. You must click [OK] to
capture the new settings.
If you click [Cancel], the instrument returns to the Experiment Window without
making any modifications.
54 FlexStation II Operator’s Manual – Rev. D