Molecular Devices FlexStation II, Руководство оператора

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1 System Description
1.6.3 Modes of Operation
The instrument operates in one integrated fluidics/read (Flex) mode and four read-only
modes. The table below compares the types of operation and features that are available in
the different operating modes.
Note: This operator’s manual describes instrument behavior for Flex mode primarily.
Refer to the SOFTmax PRO User’s Manual for instructions on other operating modes.
Flex Mode
The fluidics module for is designed to aspirate fluids from a compound source plate and
dispense them into an assay plate. Fluid transfer is made possible with an eight-channel
pipettor that is fully automated, including changing the tips from a tip rack.
In Flex mode, one to eight or one to 16 wells in one column of the assay plate are read
repeatedly for a selected total experimental time. At a preselected point or points during
that time sequence, the pipettor may transfer up to three reagents from the compound
plate to the assay plate. The instrument continues to read at the preselected time
intervals before and after fluid transfer. After completion of reading the column (or
partial column) for a preselected time, the instrument can repeat this cycle with other
columns. All the data is collected in one data file represented as a 96- or 384-well plate.
For example, an experiment with a two-minute run time will accommodate a 96-well
plate in about 24 minutes.
Run time × Number of columns = Plate time
2 minutes × 12 columns = 24 minutes
The source for transferred reagents can be either a compound plate or reservoir trough
located in the fluidics module.
24 FlexStation II Operator’s Manual – Rev. D