Molecular Devices FlexStation II, Руководство оператора

Страница: 77 / 134

3.5 Configuring Instrument Settings in Flex Mode
Manually Assigning Tips and Compounds
Alternatively to automatic assignment, you may assign any tip column and any
compound column to correspond to any assay plate column.
To assign a tip to an assay plate well, highlight one or more tip cells and select one of the
items available in the Tips pop-up menu. The tip column selected in the pop-up menu
will be shown as a number in the selected Tip row(s). Choosing the Fill option at the top
of the menu fills the selected cells with the tip numbers that correspond to the same
column numbers (tip 3 with column 3, etc.). Note that Fill does not take into account
the actual tips that are available if less than a full rack is used, whereas the choices in the
menu beneath Fill represent the actual tip numbers that are listed as being available in
the Pipette Tips Layout tab.
Similarly, to assign a compound, highlight one or more compound cells and select one of
the items from the Compound pop-up menu.
The wells shown in this settings window convey graphically the volumes of liquid in the
assay plate for each pipetting event. Using the color associated with each event, the
compound settings display the dispensed compound volume as a percentage of the total
assay well volume. If you entered an initial volume (in the Compound Transfer settings
window), that will be shown as a gray fill. As the liquid volumes are cumulative, the first
event’s volume is shown above the initial liquid volume (if any), the second event’s
volume will be shown above the first, and the third event’s volume above the second.
To deselect a tip or compound assignment, first select the appropriate cell(s) and then
press Backspace on the computer keyboard. To change an assignment, select the well(s)
and choose new values or type a value on the keyboard.
Important! If you have multiple wells selected, and you type a value, that value will be
shown in the first selected well and subsequent wells will increment to the next higher
value. Again, this does not take into account the actual pipette tips shown to be available
in the Pipette Tips Layout selection.
FlexStation II Operator’s Manual – Rev. D 71