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HiSeq X System User Guide
67
Pause or End a Run on the HiSeq X
Options to pause or end a run on the HiSeq X differ from other HiSeq instruments. Ending
a run does not provide the option to save data or resume the run.
Pause a Run
Pause a run from the Run Overview screen. Pausing a run might be necessary to check run
components or set up a run on the adjacent flow cell. For more information, see
Runs on Flow Cell A and Flow Cell B
Temporarily pausing a run allows the run to resume without loss of data. RTA v2 resumes
automatically if a run is paused. For more information, see
.
1
From the Run Overview screen, select
Pause
. The pause menu opens.
Figure 29
Pause Options
2
Select
Normal Pause
.
3
Select
Yes
to confirm the pause command. The software completes the current
chemistry or imaging command and places the flow cell in a safe state.
4
Select
Resume
to resume the run.
Change Reagents During a Run
If you started the run with a partial volume of reagents, use the Change Reagents feature to
pause the run and replenish reagents.
1
From the Run Overview screen, select
Pause
. The pause menu opens.
2
Select
Change Reagents
.
3
Select
Yes
to confirm the pause command. The software completes the current
chemistry or imaging command, places the flow cell in a safe state, and opens the
Reagents screen.
4
On the Reagents screen, enter the following reagent parameters:
•
The reagent kit ID for new reagents.
•
The number of cycles the reagents are expected to last.