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HiSeq X System User Guide
3
HiSeq X Components
The HiSeq X system comprises the instrument, monitor, instrument control computer, and
accessories, such as a keyboard, mouse, and barcode scanner. The instrument includes 4
main compartments: the optics module, flow cell compartment, fluidics compartment, and
reagents compartment. Instrument operating status is indicated on an illuminated status
bar.
Figure 1
External Components
A
Optics module
—Contains optical components that enable dual surface imaging of the
flow cell, imaging A, C, G, and T at the same time using epifluorescence. The excitation
laser beam passes through the objective and the fluorescence is collected through the
same objective.
B
Flow cell compartment
—Contains the vacuum-controlled flow cell stage, which holds
the flow cell in place during sequencing runs.
C
Fluidics compartment
—Contains fluidics pumps that deliver reagents to the flow cell,
and then to the waste container.
D
Status bar
—Uses 3 colors to indicate instrument status. Blue indicates that the
instrument is running, orange indicates that the instrument needs attention, and green
indicates that the instrument is ready to begin the next run.
E
Reagent compartment
—Contains reagent racks that hold reagents for sequencing runs
and wash solution for instrument washes.