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HiSeq X System User Guide
45
Final Water Wash
1
Load the instrument with laboratory-grade water as follows:
a
Fill 8 SBS bottles with at least 20 ml of laboratory-grade water.
b
Fill 10 PE tubes with 10 ml laboratory-grade water.
CAUTION
Do not reuse the same water or wash bottles used for the first wash step. The water from the
first wash step might be contaminated with reagents that were present on the sippers.
2
Load the bottles and tubes onto the instrument in the appropriate reagent rack.
3
Select
Next
to start the final water wash.
4
When the final water wash is complete, measure the delivered volume.
Positions
Delivered Volume
8 SBS positions
32 ml
8 SBS positions and 10 paired-end positions
72 ml
5
Unwrap the waste tubing and return the tubing to the waste bottle.