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36
Part # 15050091 Rev. C
Figure 22
Flow Cell Lever in Position 2
5
Make sure that the
Vacuum Engaged
checkbox is selected on the Load Sequencing
Flow Cell screen.
Confirm Proper Flow
Checking for proper flow confirms that the flow cell and gaskets are properly installed and
the manifold is engaged.
1
Select solution 5 from the drop-down list.
2
Enter the following default values:
•
Volume:
250
•
Aspirate Rate:
250
•
Dispense Rate:
2000
3
Select
Pump
.
4
Visually inspect the flow cell for bubbles passing through the lanes or leaks near the
manifolds.
If bubbles are present, check the manifold gaskets for obstructions and repeat the
process using solution 6 to avoid depleting position 5. Reduce the aspirate rate to 100,
and pump another 250 µl to the flow cell.
5
Select
Next
. Make sure that the flow cell lever is green, and then close the flow cell
compartment door.
6
Confirm that the checkboxes
Vacuum Engaged
and
Door Closed
are selected, and then
select
Next
.
7
Select
Start
to start the sequencing run.