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Part # 15050091 Rev. C
Introduction
The HiSeq X
™
system combines innovative engineering with proven SBS technology and
the power to enable population-scale human whole-genome sequencing.
The HiSeq X includes the following features:
}
Dual-surface imaging
—The HiSeq X uses a 2-camera, 4-sensor epifluorescence system
with cutting-edge scanning technology to enable dual surface imaging.
}
Patterned flow cell
—The HiSeq X uses a patterned flow cell that allows the generation
of sequencing clusters in an ordered arrangement, which increases output reads and
data generated.
}
High-capacity reagent chiller
—The reagent compartment is a high-capacity chiller that
holds enough reagents for the entire sequencing run.
}
Integrated fluidics for paired-end runs
—Integrated paired-end fluidics provide
reagents from the reagent compartment to the flow cell for Read 2 resynthesis and for
indexed sequencing.
}
Interface control options
—The instrument software interface provides options for
setting up a run and operating the instrument using the touch screen monitor or the
integrated keyboard.
}
Real-time base calling
—The instrument software extracts intensities from images and
performs quality-scored base calling on the instrument computer, which allows
monitoring of quality metrics during the run and saves time during subsequent data
analysis.
Downstream analysis of sequencing data can be performed with Illumina analysis
software or third-party software on a custom infrastructure.
}
BaseSpace connectivity
—The HiSeq X features an option to send instrument health
and sequencing data to the BaseSpace genomics cloud solution in real time to
streamline instrument quality control and analysis.