P
o
st
-R
u
n
P
ro
c
e
d
u
re
s
46
Part # 15050091 Rev. C
Perform a Water Wash
After each sequencing run, an instrument wash is required. You have the option to perform
a maintenance wash or a water wash after a run. Illumina recommends a maintenance
wash after each run.
NOTE
Using HiSeq X Control Software v3.1, or later, a maintenance wash is recommended after
each run, but no longer required.
If the instrument has been idle for 1 day or more, perform a water wash before beginning a
new sequencing run.
Illumina-Supplied Consumables
}
Laboratory-grade water
User-Supplied Consumables
}
8 bottles, 250 ml (Corning, catalog # 430776)
}
10 tubes, 15 ml (Corning, catalog # 430052)
}
Laboratory-grade water
Procedure
1
From the Welcome screen, select
Wash | Water
.
2
Select
Yes
to wash paired-end reagent positions. Otherwise, select
No
to wash
SBS reagent positions only. Select
Next
to proceed.
3
Load the instrument with laboratory-grade water as follows:
a
Fill 8 SBS bottles with 20 ml laboratory-grade water.
b
Fill 10 PE tubes with 10 ml laboratory-grade water.
4
Make sure that a used flow cell is loaded. Load a used flow cell, if necessary. Select
Next
.
5
Perform a fluidics check:
a
Select solution 2 from the drop-down list. Accept the default pump values.
b
Select
Pump
.
c
Visually inspect the flow cell for bubbles passing through the lanes and leaks near
the manifolds.