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32
Part # 15050091 Rev. C
Position Tubing and Start Prime
1
Remove the waste tubing for each flow cell from the waste container.
Do not remove the tubing for the condensation pump.
Figure 16
Position Tubing
2
Place each waste tubing into a separate empty 15 ml tube. Priming waste is collected
and measured after the priming step.
3
Select
Start Prime
. The priming screen opens and the priming step begins. Monitor the
progress of the priming step from the priming screen.
4
When the priming step is complete, measure the collected priming volume and confirm
that the volume in each tube is
1.75 ml
.
If you collect priming volumes in 1 bottle for each flow cell, confirm that the volume is
14 ml
.
Volumes are calculated as follows:
•
250 µl for each SBS position except position 2 (250 x 7 = 1.75 ml)
•
1.75 ml for each lane (1.75 x 8 = 14 ml)
5
Record the results on the lab tracking form.
6
Return the waste tubing to the waste container before proceeding.
7
Select
Next
. You are ready to load the clustered flow cell.