illumina HiSeq X, Руководство пользователя, Страница: 37 / 86

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HiSeq X System User Guide
27
5 Remove the caps from each reagent tube and place the tube onto the rack in the
associated numbered position or matching label color.
Position
Reagent Description
10
PRM Patterned Resynthesis Mix
11
PLM2 Patterned Linearization Mix 2
12
PW1 10 ml PW1 or laboratory-grade water
13
PAM Patterned Amplification Mix
14
PPM Patterned Amp Premix
15
PDR Patterned Denaturation Mix (contains formamide)
16
HP11 Primer Mix, Read 2
17
HP12* Primer Mix, Index 1 Read
18
PW1 10 ml PW1 or laboratory-grade water
19
PW1 10 ml PW1 or laboratory-grade water
Table 2 Paired-End Reagent Positions
*HP12 is required for indexed runs only. If HP12 is not used, load a 15 ml conical tube with 10 ml PW1
or laboratory water.
6 Slide the reagent rack into the reagent compartment, aligning the rack with the raised
guide on the floor of the compartment.
7 Lower the sippers into the paired-end reagent tubes as follows:
a Pull the handle towards you and then lower the handle.
b Visually inspect the sippers to make sure that they do not bend as they lower into
the tubes.
c Release the handle into the slot on the bottom end of the groove.
8 Select Next .
Prime Reagents
Steps for priming reagents include cleaning the flow cell holder, loading a priming flow
cell, confirming proper flow, and then starting the prime.
Clean the Flow Cell Holder
1 Open the flow cell compartment door.