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HiSeq X System User Guide
31
Figure 15
Flow Cell Lever in Position 2
7
Make sure that the
Vacuum Engaged
checkbox is selected on the load prime flow cell
screen, and then select
Next
.
Confirm Proper Flow
Checking for proper flow confirms that the flow cell and gaskets are properly installed and
the manifold is engaged.
1
Select solution 2 (laboratory-grade water) from the drop-down list.
CAUTION
Use water to confirm proper flow on a used flow cell only. Never use water to confirm
proper flow on a clustered flow cell.
2
Confirm the following default values:
•
Volume:
125
•
Aspirate Rate:
250
•
Dispense Rate:
2000
3
Select
Pump
.
4
Visually inspect the flow cell for bubbles passing through the lanes and leaks near the
manifolds.
If excessive bubbles are present, check the gaskets for obstructions, reduce the aspirate
rate to 100, and pump another 125 µl of water to the flow cell. If problems persist,
remove the flow cell, repeat the cleaning steps, and reload the flow cell.