GE Typhoon 9200, Руководство по эксплуатации

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4 Operation
4.3 Preparations before start
30
Typhoon Operating Instructions 28-9623-56 AA
Storage phosphor screen sample handling recommendations
• Protect a general-purpose (GP) screen from contamination by separating a wet
gel from the screen with a piece of plastic wrap or polyester film. Wrap the plastic
wrap completely around the gel so that liquid cannot leak out.
This precaution reduces screen contamination with minimum attenuation of the
signal.
• Do not use scintillants or enhancers, such as PPO, EN3HANCE™, and Amplify™.
These products interfere with the proper function of the screen.
• For recommendations of use of radioactive standards, see Typhoon User´s Guide.
Fluorescent sample handling recommendations
• Always rinse the gloves with distilled or tap water before handling the sample and
before preparing the reagent.
• Handle filter solutions as follows
• Remove dust particles by filtering all the stock solutions used to prepare
the sample, sample matrix, and buffers.
• Use clean, rinsed containers.
• When diluting clean reagents to working concentration, use distilled
water collected in a rinsed container so that you do not have to filter the
solutions again.
• If available use dust free reagents, such as fluorescent labels. They require
no further filtration.
• Avoid fluorescent indicator dyes.
Many of the commonly used electrophoresis tracking dyes fluoresce. Whenever
NOTICE
Do not use wet gels in combination with tritium (TR) screens.
NOTICE
Do not directly expose the Storage phosphor screen to wet chemicals of
any kind, especially not to organic solvents.
Since organic solvents penetrate plastic, plastic wrap will not protect the
Storage phosphor screens from organic solutions.
NOTICE
Neutralize alkaline denaturing gels and make sure the sample is free
from acetic acid vapors and organic solvents.
These materials are harmful to the Storage phosphor screens and can
penetrate plastic wrapping.