GE Typhoon 9200, Руководство по эксплуатации

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4 Operation
4.3 Preparations before start
32
Typhoon Operating Instructions 28-9623-56 AA
Table 4-1. Material recommendations for low-fluorescent sample handling.
Chemiluminescent sample handling recommendations
• Follow the manufacturer’s guidelines for preparing membranes or microplates for
chemiluminescence scanning.
• To avoid compromising the chemistry on the membrane, place the membrane
between two pieces of transparency material or between sheet protectors.
Alternatively, place the membrane in a plastic bag to keep the sample wet and
surrounded by substrate.
Material Recommendation
Gels, general Background fluorescence contributed by the gel matrix
increases with gel thickness. Always use the thinnest gel
practical for your experiment, especially for agarose gels.
Make sure your glass plates are absolutely clean before you
pour the gel. Grease and fingerprint oils from the plates can
stick to the gel surface and attract dust and fluorescent dyes.
Agarose gels Make sure the agarose is completely dissolved and well-mixed
before pouring your gel. Also, avoid generating bubbles when
mixing and pouring. Uneven agarose concentration and
bubbles affect light scatter and can cause artifacts and
interfere with quantitation.
Polyacrylamide
gels Polyacrylamide gels are usually clear and thin (less than 1
mm). The background contribution from the gel material is
generally very low.
Solvents Spectroscopic-grade solvents have the lowest
autofluorescence.
Membranes You should scan a test piece of each type of membrane you
plan to use and check that the background is low enough for
your purposes.
Transparency
support To avoid contaminating the glass platen and sample lid, you
can place a sample, such as a membrane, between two pieces
of transparency material. For detailed instructions, see the
Typhoon User´s Guide .