GE Typhoon 9200, Руководство по эксплуатации

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Typhoon Operating Instructions 28-9623-56 AA
31
Operation 4
Preparations before start 4.3
possible, put the tracking dye in a separate lane. Alternatively, dilute the indicator
dyes as much as possible.
• Avoid excessive exposure to light.
Fluorochromes differ greatly in their sensitivity to light-induced degradation
(photobleaching). When working with sensitive fluorochromes and fluorescently
labeled samples, use low-light conditions.
Recommendation for selecting the label for the fluorescence scanning
• Green-excited fluorescence
Detects samples labeled with fluorochromes that are excited at 532 nm.
• Red-excited fluorescence
Detects samples labeled with fluorochromes that are excited at 633 nm.
• Typhoon 9400, 9410, Trio and Trio+: Blue-excited fluorescence
Detects samples labeled with fluorochromes that are excited at 457 (Typhoon
9400 and 9410) or 488 nm (Typhoon 9400, 9410, Trio and
Trio+ ).
Note: The laser power at 457 nm is much lower than the laser power at 488 nm.
• Multilabel fluorescence
Detects samples labeled with two, three, or four fluorochromes and creates a
multichannel image. To minimize the spectral overlap from the fluorochromes, the
emission peaks should be greater than 30 nm apart. Use one or more lasers and
up to four different emission filters to create the multichannel image. The
multichannel image contains one image for each fluorochrome. Use analysis
software to view these images separately or overlaid.
Note: The instrument contains a built-in filter for the red and green lasers. The built-
in filter blocks the laser light at 532 nm ±8 nm or 635 nm ±10 nm. For the
Typhoon 9400 and 9410 instruments, an additional built-in filter blocks all
laser light below 500 nm. The Typhoon system automatically selects the
appropriate built-in filter based on the color of the laser you selected. The
built-in filters cannot be used simultaneously.
Low-fluorescent sample handling recommendations
For fluorescent samples requiring high sensitivity or highly accurate quantitation, the
material sources stated in
Table 4-1 have been tested and found to have low
background.