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BioTek Instruments, Inc.
Evacuation Efficiency Test (4 Well Manifold)
Important: You must create your own qualification test protocols for this
manifold type:
See Create Qualification Protocols for 4 Well Manifold on
page 20.
Make sure the Instrument>Manifold setting matches the installed 4 Well
manifold.
If you tared the balance at the start of the Dispense Precision Test (on
page page 18) you can use the plate from that test here;
skip steps
1-3
.
1.
Fill a supply bottle with approximately 250 mL of
deionized water
. Run
Maintenance program
DAY_RINSE
to prime the fluid lines and manifold.
2.
Place a new 24-well microplate on the balance and zero the balance.
3.
Pipette or dispense 1120 μL of
Solution #1, 2, or 3
(your choice) into each
well of the microplate.
4.
Place the plate on the washer and run the protocol created for the test, e.g.
4-Well residual_qc_test
.
5.
When the program is finished, remove the plate and weigh it
immediately, because evaporation will affect the results. This is the
Total
Residual Weight
,
in grams.
6.
Visually inspect the plate and note if any wells appear to have
considerably more liquid in them than others.
7.
Using the worksheet at the end of this chapter, calculate results:
o
Divide the Total Residual Weight by
24
to find the
Mean Residual
Weight
.
o
The Mean Residual Weight should be
<= 0.05 g
If the Mean Residual Weight is greater than 0.05 g, or if one or more wells
appear to have much more liquid than the others, the washer failed the test.
If the test fails once, troubleshoot as follows:
•
If the problem appears to be related to particular wells, clean those
aspiration tubes:
Clean the Aspirate and Dispense Tubes
. When finished,
retry the test.
•
Failure may occur when a microplate other than the recommended 24-well
Corning 3524 plate is used. Modify the aspirate height (Z offset) or other
parameters to accommodate the different plate geometry. After modifying
the protocol, retry the test using a new microplate.
