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BioTek Instruments, Inc.
Method
Action/Description
Default
and 2x8-well manifolds), where 1 = Slowest, 9 = Fastest. See
Dispense and Prime Flow Rates on page 1
.
Dispense Protocol Parameters
Options
Actions
Default
Dispense Volume
The volume to dispense per well per wash cycle. The
volume range is 50 to 3000 µL/well for 96-well plates.
When dispensing to 384-well plates, the range is 25-
3000 μl/well.
300
Dispense Flow Rate
Flow rates range from 1 to 9 (except 1-7 for the 12-
channel manifold, and 1-5 for the 4 and 2x8 Well
manifolds). 1 = Slowest, 9 = Fastest.
See Dispense
and Prime Flow Rates on page 1
. The flow rate must be
valid for the manifold and dispense volume. For
Troubleshooting:
See Dispense Volume Invalid for
Manifold Type (0F00 Error)
.
5
Dispense Height (Z-
axis)
The height between the bottom of the aspirate tubes
and the carrier surface when dispensing. The dispense
tubes are shorter than the aspirate tubes.
The Dispense Height range is from 12 to 180 steps.
The washer displays the corresponding millimeters. 12
is closest to the carrier surface, 180 is furthest from
this surface.
Overflow Position: It is considered good practice to set
a dispense height that positions the aspirate tubes at
the top of wells to draw off any overflow.
120
Horizontal Position
(Y-axis)
The position of the manifold tubes in relation to the
center of the microwells.
The Horizontal Disp Pos range is from -24 to 24 for the
8- and 12-channel manifolds and the 2x8 manifold; -88
to 24 for the 8s-manifold; -24 to 15 for the 16-channel
when addressing 96-well plates and -22 to 10 for 384-
well plates; the 4 (24-well plate) manifold range is -
180-60. The washer displays the corresponding
millimeters.
A negative offset moves the dispense tubes back, away
from the front of the washer. A positive offset moves
the dispense tubes toward the front of the washer. A
setting of 0 indicates no offset.
0
