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BioTek Instruments, Inc.
Dispense Precision Test (8, 8s, 2X8, and 12 Well)
For washers with
Buffer Switching
the predefined protocols provided
are set to use Buffer “
A
.” If you would prefer to use a different buffer,
create your own dispense test protocol using the Liquid Test Protocols:
Default Parameters on page 7. Prime the system with the selected
“Buffer” before running tests if the washer has been idle.
When this test is complete, the filled plate can be used to perform the
Evacuation Efficiency Test.
1.
Use a clean, dry 96-well microplate. If you will be using the same plate for
the
Evacuation Efficiency Test
: place a new plate on the balance and tare
the balance.
2.
Prepare the washer to dispense test
Solution #3
to the microplate: fill a
supply bottle and prime the tubing and the manifold
(
Quick>Prime>System
).
3.
Run the dispense program
accuracy_qc_test
. Enter the number of strips
to process (
12
for the 8-/8s- and 2X8Well manifolds; or
8
for the 12 Well
manifold).
4.
Read the plate in an absorbance reader (blank on air) using the dual-
wavelength method (630-450 nm). Print or export the results.
Note: If you are using one of BioTek’s keypad-based readers, such as
the ELx800™ or ELx808™, ensure that the reader is not running in
Rapid mode. To check this, select
U T I L > R E A D
and cycle through the
prompts until you see
R E A D I N R A P I D M O D E ?
, then choose
N O
for
an accurate result.
5.
Calculate and report the mean absorbance, standard deviation, and
% Coefficient of Variation (%CV) for the plate.
Performance specifications
— a %CV greater than:
•
3.0% is a failure for the 8, 8s, and 12 manifolds
•
4.0% is a failure for the 2X8 manifold
If your %CV result fails, clean the dispense tubes with a stylus and retest.
