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BioTek Instruments, Inc.

Protocol Parameters Reference Tables

Review these tables for details about the available protocol parameters:

Wash Protocol Parameters on

page 16

Dispense Protocol Parameters on

page 17

Aspiration Protocol Parameters on

page 19

Soak and Shake Protocol Parameters on

page 20

Define: Wash Parameter Categories

When creating or editing a wash protocol, parameters are organized by

the following categories:

Step

Parameters

METHOD Wash cycles, plate or strip format, soaking and shaking, soak duration,

shake before soak, shake duration, shake intensity, prime after soak,

prime volume and prime flow rate.

DISP

Dispense Volume, Dispense Flow Rate, Dispense Height, Horizontal

Dispense Position, Bottom bv Wash First, Bottom Dispense Volume,

Bottom Flow Rate, Bottom Dispense Height, Bottom Horizontal Position,

Prime Before Start, Prime Volume, Prime Flow Rate.

ASPIR

Aspirate Height, Horizontal Aspirate Position, Aspirate Rate, Aspirate

Delay, Crosswise Aspirate, Crosswise On, Crosswise Height, Crosswise

Horizontal Position, Final Aspiration, Final Aspirate Delay.

Содержание 50 TS

Страница 1: ...50 TS Washer Instructions for Use For In Vitro Diagnostic Use April 2017 April 2017 Part Number 1551010 Revision A BioTek Instruments Inc ...

Страница 2: ...S Washer is a trademark of BioTek Instruments Inc Microsoft Internet Explorer Windows Windows 7 Windows 10 and Excel are either registered trademarks or trademarks of Microsoft Corporation in the United States and or other countries All other trademarks are the property of their respective holders Restrictions and Liabilities Information in this document is subject to change and does not represent...

Страница 3: ...www biotek de Kocherwaldstrasse 34 Phone 49 0 7136 9680 D 74177 Bad Friedrichshall Fax 49 0 7136 968 111 Germany E Mail info biotek de Instructions for Use Requirements This document fulfills the basic needs of persons operating this device according to the requirements of the In Vitro Diagnostic Directive for Instructions for Use Some of the device s higher level functions and features as well as...

Страница 4: ... insert for the test to be conducted Failure to conduct Quality Control checks could result in erroneous test data Warnings Operate the instrument on a level stable surface away from excessive humidity When operated in a safe environment according to the instructions in this manual there are no known hazards associated with the 50 TS Washer However the operator should be aware of certain situation...

Страница 5: ...o the external power supply Use of an adapter disconnects the utility ground creating a severe shock hazard Always connect the power cord directly to an appropriate receptacle with a functional ground Warning Service Only qualified technical personnel should perform service procedures on internal components Warning Accessories Only accessories which meet the manufacturer s specifications shall be ...

Страница 6: ... Quality Control The operator must follow the manufacturer s assay package insert when modifying software parameters and establishing washing or dispensing methods Failure to conduct quality control checks could result in erroneous test data Warning Internal Voltage Always turn off the power switch and unplug the power supply before cleaning the outer surface of the instrument Warning Potential Bi...

Страница 7: ...cals may cause irreparable damage to the instrument The following chemicals have been deemed safe for use in the instrument buffer solutions such as PBS saline surfactants deionized water 70 ethyl isopropyl or methyl alcohol 40 formaldehyde and 20 sodium hydroxide Never use acetic acid DMSO or other organic solvents These chemicals may cause severe damage to the instrument Contact BioTek for more ...

Страница 8: ...erein this instrument bears the CE mark Note See the Declaration of Conformity for specific information Directive 2014 30 EU Electromagnetic Compatibility Emissions Class A The system has been type tested by an independent accredited testing laboratory and found to meet the requirements of EN 61326 1 Class A for Radiated Emissions and Line Conducted Emissions Verification of compliance was conduct...

Страница 9: ...ntrol and laboratory use Part 1 General requirements EN 61010 2 081 Particular requirements for automatic and semi automatic laboratory equipment for analysis and other purposes Directive 2012 19 EU Waste Electrical and Electronic Equipment Disposal Notice Dispose of the instrument according to Directive 2012 19 EU on waste electrical and electronic equipment WEEE or local ordinances Directive 98 ...

Страница 10: ...nsultare la doc annessa Warning risk of electric shock Attention risque de choc électrique Gefährlicheelektrischeschlag Precaución riesgo de sacudidaeléctrica Attenzione rischiodiscossaelettrica Warning risk of crushing or pinching Attention risqued écrasement et pincement Warnen Gefahr des Zerquetschens und Klemmen Precaución riesgo del machacamiento y sejeción Attenzione rischiodischiacciareedin...

Страница 11: ... xi BioTek Instruments Inc Consult instructions for use Consulter la notice d emploi Gebrauchsanweisung beachten Consultar las instrucciones de uso Consultare le istruzioni per uso ...

Страница 12: ...on This section provides detailed installation instructions Unpack and Inspect the Instrument 2 Setting Up the 50 TS 3 Connect the Fluid Supply and Waste System 4 Install the Microplate Carrier 13 Attach the Mist Shield 15 ...

Страница 13: ...arrier and your BioTek representative Keep the shipping cartons and packing material for the carrier s inspection BioTek will arrange for immediate repair or replacement of your instrument Unpack the accessories and the washer Washers are shipped with two or three 2 liter supply bottles and one 2 liter waste bottle The 50 TS with Buffer Switching V version includes three supply bottles Liquid Leve...

Страница 14: ...rtant The manifold dispense tubes have a protective Teflon collar at the tip This is to prevent dripping Do not remove these coverings Operating Environment The 50 TS washer is sensitive to extreme environmental conditions For optimal operation install the washer on a stable level surface in an area where ambient temperatures between 15 40 C 59 104 F can be maintained and away from excess humidity...

Страница 15: ...les the liquid level alert bottles have a sensing device inside connected to an electrical cable in the top When the washer is installed follow the instructions to enable the system See Install the Liquid Level Alert System Optional on page 9 Connect the Fluid Supply Fluid Supply without Buffer Switching is shown in this photo The tubing connects directly from the bottle to the syringe pump to the...

Страница 16: ... supply Input Make sure the flow arrow points toward the washer 5 Without Buffer Switching Connect the long tube to the top of the supply bottle With Buffer Switching The tube will extend only from the syringe pump to the Valve Output port next to the pump 1 First remove the Luer fitting on one end of the tube and cut the long tube to about 5 12 7 cm from the check valve 2 Reinsert the Luer fittin...

Страница 17: ...6 50 TS Washer 5 Connect the three supply bottles A B C to their respective ports ...

Страница 18: ...8MF 8M 8F Connecting the Waste system tubing on the back of the washer 1 Remove the waste bottle cover and replace it with the stopper from the waste tubing set 2 Attach the Y shaped tubing from the waste bottle stopper to the two waste ports on the back of the wash manifold 3 Install the inline vacuum filter ...

Страница 19: ...from escaping into the air and 2 to serve as a temporary fluid barrier if the waste bottle is allowed to overfill 4 After inserting the inline vacuum filter Connect the tubing from the waste bottle to the Vacuum port on the back of the washer 50 TS 8F 8M 8MF models for vacuum filtration Installing the Waste system for vacuum filtration models 8F 8M and 8MF models 1 Attach the Y shaped tubing to th...

Страница 20: ...ective ports on the back of the washer Vacuum port to connect the vacuum pump to the waste bottle Waste port to capture waste fluid from the washer 6 Connect the tubing from the vacuum filtration carrier to the Carrier port on the back of the washer Disconnect the waste tube from the Carrier port when you are not using the vacuum filtration carrier An internal valve preserves the vacuum required t...

Страница 21: ...supply bottle cable to port A and then insert the shorting plugs into ports B and C o For all washers connect the waste bottle cable to the WASTE port 3 Connect the select box to the LIQUID ALERT port on the back of the washer 4 Enable Liquid Level Alert by selecting Instrument Options and making Liquid Level Alert Enabled Liquid Level Alert Setup Install Vacuum Pump Muffler Optional Install the o...

Страница 22: ...uter posts and secure with thumbscrews 1 Orient the manifold with the aspirate and dispense tubes facing down and the fittings for the supply and waste tubing facing the back of the washer 2 Place the manifold gently onto the two support pins on the manifold mounting bracket closest to the front of the washer 3 Insert the thumbscrews 2 Do not overtighten See also Connect the Fluid Supply and Waste...

Страница 23: ...nd dispense tubes facing down and the fittings for the supply and waste tubing facing the back of the washer 2 Place the manifold gently onto the three support pins on the manifold mounting bracket 3 Insert the thumbscrews 3 Do not overtighten See also Connect the Fluid Supply and Waste System on page 4 for descriptions of the manifold tubing connections ...

Страница 24: ...o the priming trough is closest to the rear of the washer 2 Line up the pin on the underside of the carrier with the slot on the carrier transport rail and install the carrier Rail guides under the carrier sit on rail Standard microplate carrier with plate Carrier base for biomagnetic separation and vacuum filtration plate carriers ...

Страница 25: ...base 2 Observe on the underside of the vacuum filtration and biomagnetic separation carriers the holes for pins in each of the four corners Align the carrier to fit on top of the pins in the carrier base 3 Vacuum filtration carrier connect the tubing on the special plate carrier to the Carrier port on the back of the washer The biomagnetic separation carrier s priming trough is removable Make sure...

Страница 26: ...d during installation 2 Make sure the fluid supply and the waste output tubes are attached to the appropriate manifold ports Lift the manifold mounting bracket up or back to make sure the tubing to the manifold is not being pulled too tightly 3 Vacuum Filtration models make sure there is sufficient room behind the washer for the waste tubing attached to the special carrier to move during operation...

Страница 27: ...ws into the slots on the washer s left side Do not tighten completely 2 With the door toward the front of the washer slide the mist shield down onto the thumbscrew shafts while inserting the two pins into the slots on the top left side 3 Tighten the thumbscrews ...

Страница 28: ... supply Use of a two prong adapter disconnects the utility ground creating a severe shock hazard Always connect the power cord directly to a three prong receptacle with a functional ground The 50 TS Washer uses an external 24 volt power supply The power supply automatically adjusts for input voltage in the range of 100 250 V 1 Plug the power supply s plug into the washer s rear panel 2 Insert the ...

Страница 29: ...this chapter and before the instrument is used in a laboratory environment The successful completion of the Installation Qualification confirms that the washer and its components have been supplied as ordered and ensures that they are assembled and configured properly for your lab environment The successful completion of the Operational Qualification confirms that the washer is operating according...

Страница 30: ...asics of operating the 50 TS For more detailed and comprehensive instructions please refer to the operator s manual AutoPrime 2 Wash a Plate 4 Optimize Performance 7 Run a Predefined Protocol 8 Create a Protocol 13 Instrument Configuration Setup Controls 23 ...

Страница 31: ...st precise dispense volumes always run a Quick Manifold Prime before a basic wash or dispense Buffer Switching models may require a System prime if the instrument has been idle AutoPrime LHC Tools Instrument Utilities AutoPrime From the Home screen Instrument AutoPrime Recommended for optimum performance AutoPrime keeps the tubing wet in between runs and can be an essential part of your daily main...

Страница 32: ... AutoPrime Touchscreen LHC 1 Select Instrument at the main menu and select AutoPrime 1 Select Tools Instrument Utilities AutoPrime tab 2 Enable AutoPrime and specify the parameters the idle time interval that will trigger priming up to 24 hours Set the Submerge Duration if desired Flow rate volume and buffer valve if applicable 3 LHC users click Send to transfer the settings to the instrument ...

Страница 33: ...common daily uses System to prime all the tubing when changing buffers for example and Manifold to prime the dispense tips to correct for evaporation Basic Wash after priming the tubing specify the volume per well buffer bottle if applicable number of wash cycles and how many strips or rows to wash When you are ready to wash a plate 1 Fill the supply bottle and connect it to the washer empty the w...

Страница 34: ...of the aspirate tubes more or fewer wash cycles or a slower aspirate travel rate would improve your results e g reduce residuals use the Protocol menu to customize a protocol for your assays Several sample protocols are provided which can be used as templates to give you shortcut to creating your own protocols Copy a sample protocol to preserve the original and start the customization with optimiz...

Страница 35: ...ate step You can customize the wash protocol o Change the number of cycles o Add pre wash steps like bottom wash for very vigorous washing o Remove the final aspirate step and raise the aspirate height to increase residuals in the wells o Shake the plate to mix the contents o Add a Soak or delay to incubate the contents at room temperature o Etc See Run a Predefined Protocol on page 8 See Create a...

Страница 36: ... more than three quarters full Alternatively install the Liquid Level Alert Check the external tubing connections for kinks and clogs Make sure the bottles solutions and tubing are clean and do not contain any particles or mold To avoid creating air bubbles every time the supply bottle is filled make a mark halfway down the bottle and refill when the fluid level drops to that point Unscrew the cap...

Страница 37: ...ove aspirate excess fluid during the dispense step If you use more than one manifold make sure the instrument s setting matches the installed manifold and plate carrier Run a Predefined Protocol BioTek ships the 50 TS with several predefined protocols onboard See Predefined Onboard Protocols Listing on page 9 Certain prompts are displayed when a protocol is selected for run Obey the prompts by mak...

Страница 38: ...50 TS models Run predefined protocols from the Main Menu Home Protocol settings have been optimized based on experience in our applications lab Sample Wash Protocols for all 50 TS Models with 8 8s and 12 Well Manifolds Protocol Name COSTAR_FLAT COSTAR_ROUND NUNC_FLAT NUNC_ROUND Plate Type 96 96 96 96 Number of Cycles 3 3 3 3 Wash Format Plate Plate Plate Plate Soak After Dispense No No No No Shake...

Страница 39: ... FLT16_384 NUNC_ FLT16_96 NUNC_ FLT16_384 Plate Type 96 384 96 384 Number of Cycles 3 3 3 3 Wash Format Plate Plate Plate Plate Soak After Dispense No No No No Shake After Soak No No No No Prime After Soak No No No No Dispense Volume 300 100 300 100 Dispense Flow Rate 5 5 5 5 Dispense Height 120 120 120 120 Horizontal Dispense Position 0 0 0 0 Bottom Wash First No No No No Prime Before Start Yes Y...

Страница 40: ...mat Plate Plate Soak After Dispense Yes 30 sec Yes 30 sec Shake After Soak No No Prime After Soak No No Dispense Volume 200 100 Dispense Flow Rate 3 5 Dispense Height 120 128 Horizontal Dispense Position 0 0 Bottom Wash First No No Prime Before Start Yes Yes Prime Volume 5 5 Prime Flow Rate 5 5 Aspirate Height 29 58 Horizontal Aspirate Position 0 24 Aspirate Rate 7 1 Aspirate Delay 0 0 Crosswise A...

Страница 41: ...146 5 accur_qc_test384 100 120 6 ASPIRATE Height Z Asp Horizontal Y Travel Rate residual_qc_test 28 20 1 resid_qc_test384 22 0 3 Vacuum Filtration Volume µL well Flow Rate VAC30 300 6 Maintenance Protocols MAINTENANCE DAY_RINSE DECONTAMINATION LONG_SHUTDOWN OVERNIGHT_LOOP RINSE_AND_SOAK PRIME_ALL_BUFFERS1 1 For V models with buffer switching capability ...

Страница 42: ...See Protocol Parameters Reference Tables on page 15 1 Select Protocol at the main menu 2 Select Create 3 Enter a unique name for the new protocol 4 Select the Plate Type and press Save 5 Press Add and select the type of action to perform 6 Define the parameters for the step and add more steps as needed ...

Страница 43: ...ed or cut step then press Paste Wash Step Touch the Aspirate and or Dispense boxes to modify the default parameters for these parts of the wash step Press Wash options to enable and define additional actions Toggle the Yes No buttons to enable or disable an option Press Edit to define the parameters of the option See Wash Protocol Parameterson page 16 for descriptions In an aspirate or dispense st...

Страница 44: ...es Step Parameters METHOD Wash cycles plate or strip format soaking and shaking soak duration shake before soak shake duration shake intensity prime after soak prime volume and prime flow rate DISP Dispense Volume Dispense Flow Rate Dispense Height Horizontal Dispense Position Bottom bv Wash First Bottom Dispense Volume Bottom Flow Rate Bottom Dispense Height Bottom Horizontal Position Prime Befor...

Страница 45: ...delay processing or to steep fluid in the wells before aspiration A soak begins after the wash buffer is dispensed In some assays it enhances washing by allowing unbound material to diffuse into the wash buffer When washing strips in a plate format use a soak that lasts as long as it takes to process one wash cycle of all strips 30 Shake Duration The amount of time to agitate the plate The duratio...

Страница 46: ...surface when dispensing The dispense tubes are shorter than the aspirate tubes The Dispense Height range is from 12 to 180 steps The washer displays the corresponding millimeters 12 is closest to the carrier surface 180 is furthest from this surface Overflow Position It is considered good practice to set a dispense height that positions the aspirate tubes at the top of wells to draw off any overfl...

Страница 47: ... aspirate step is defined as VAC this aspiration method is used for the Bottom Wash as well No Bottom Disp Volume The volume range is 50 to 3000 µL well 150 400 µL well is typical 300 Bottom Flow Rate Valid options 1 to 9 except 1 7 for the 12 channel manifold and 1 5 for the 4 and 2x8 Well manifolds 5 Bottom Disp Height Valid range from 12 to 180 steps 60 Bottom Horiz Pos Valid range is same as r...

Страница 48: ... the 2x8 manifold 88 to 24 for the 8s manifold 24 to 15 for the 16 channel when addressing 96 well plates and 22 to 10 for 384 well plates the 4 24 well plate manifold range is 180 60 The washer displays the corresponding millimeters A negative offset moves the aspirate tubes back away from the front of the washer A positive offset moves the tubes toward the front of the washer A setting of 0 indi...

Страница 49: ...s useful for eliminating residual sample or reagent from the wall perimeter No Final Aspirate Select YES to designate a final aspiration leaving the wells empty Yes Final Aspirate Delay Same as Aspiration Delay see above applied to the final aspiration 0 Negative values To enter a negative value toggle the button 2 Secondary and Final Aspiration are only available in a wash step not stand alone as...

Страница 50: ...n The range is from 1 second to 30 minutes Note When washing strips in a plate format define a soak that lasts as long as it takes to process one wash cycle of all strips System Startup To turn on the 50 TS press the on off switch on the right side of the base The 50 TS performs a self test each time it is started If the self test fails the washer will beep and display an error code Note the error...

Страница 51: ...hen editing a protocol press Info to change the protocol s name or plate type How to delete a protocol Delete a protocol to remove it from the Home screen Main Menu and to free up space onboard for more protocols 1 Select Protocol at the Home screen 2 Highlight the protocol you want to remove and press Delete 3 Confirm your intention to remove the protocol at the prompt ...

Страница 52: ...pe PN Plate Type Onboard 4 Well 1550504 24 well 4 8 Well 4070512 96 well 8 8 Well Short Dispense Tube 4070519 96 well 8s 2 X 8 Well 1550501 96 well 2x8 12 Well 4070513 96 well 12 8 16 Well 4070527 96 384 well 8 16 Plate Carrier Selection The type of plate carrier installed on the instrument must match this setting to ensure expected performance Standard 384 Well compatible with the 8 16 Well manif...

Страница 53: ...checks the status of the sensor at the start and end of all runs Error codes 2800 or 2900 display when a sensor is activated If this happens press OK check the state of the supply and waste bottles You can fill or empty the bottles as needed and then continue the run if desired Sensor detection is disabled on the washer by default To change the setting select Instrument Options and set Liquid Leve...

Страница 54: ... 25 BioTek Instruments Inc wash buffers that are prone to harden or crystallize See AutoPrime on page 2 ...

Страница 55: ...aintaining the 50 TS For more detailed and comprehensive instructions please refer to the operator s manual About Maintaining the Washer 2 Recommended Maintenance Schedule 3 Daily Maintenance Rinse Soak the Fluid Path 8 Clean Components 11 Decontaminate the Washer 18 ...

Страница 56: ...ids you re using prone to dry and harden quickly If yes the dispense and aspirate tubes can clog quickly and therefore they must be rinsed frequently and cleaned regularly Is the washer in use continuously or does is it idle for several hours or days at a time If the washer will be sitting idle the tubes should be soaked to keep them in a wetted state Perform the rinsing procedure or Enable AutoPr...

Страница 57: ... RINSE_AND_SOAK As needed Protein residual removal Clean Components Clean bottles Check empty waste bottle Clean manifold Clean mist shield Clean aspirate and dispense tubes Clean microplate carrier Clean or replace check valves Decontaminate the Washer Decontaminate the external surfaces Run DECONTAMINATION Prepare the Washer for Storage or Shipment Run LONG_SHUTDOWN Replace Components Check valv...

Страница 58: ...aintenance Program RINSE_AND_SOAK Prime Volume 60 mL Flow Rate 5 Soak After Prime Yes Soak Duration 5 minutes Maintenance Program OVERNIGHT_LOOP Prime Volume 60 mL Flow Rate 5 Soak After Prime Yes Soak Duration 4 hours To keep the washer s dispense tubes wet overnight or over a weekend the Overnight_Loop protocol repeats the prime and 4 hour soak twelve times for approx 48 hours Fill the supply bo...

Страница 59: ... Prompt Connect disinfectant bottle Conn disinfect bottles to A B Prime 60 mL rate 5 soak 20 mins Buffer A 40 mL rate 5 no soak Prime Buffer B 40 mL rate 5 no soak Prompt Connect rinse bottle Disinfect to C rinse to A B Prime Buffer C 60 mL rate 5 soak 20 mins Prime 60 mL rate 5 soak 2 mins Buffer A 60 mL rate 5 no soak Prime Buffer B 60 mL rate 5 no soak Prompt Connect rinse bottle to C Prime Buf...

Страница 60: ... mL rate 5 no soak Prompt Disconnect bottle Connect rinse bottle to C Prime 60 mL rate 5 no soak Buffer C 60 mL rate 5 soak 2 mins Prompt Disconnect all bottles Prime Buffer A 60 mL rate 5 no soak Prime Buffer B 60 mL rate 5 no soak Prime Buffer C 200 mL rate 5 soak 2 mins Dead Volume Consider these approximate dead volumes when defining priming values to adequately prime the tubing before running...

Страница 61: ...ld Dead Volume 50 TS without Buffer Switching 20 mL 50 TS with Buffer Switching V models with full 28 supply tubing 35 mL 50 TS V model with supply tubing cut to 8 25 mL All manifolds except the 2x8 Well manifold 5 mL 2x8 Well manifold 10 mL ...

Страница 62: ...m 1 Fill the supply bottle with an appropriate cleaning agent for the fluids you have been dispensing 2 Select Maintenance from the Main Menu 3 Select the desired maintenance program 4 Press START to begin To stop a program in progress press STOP 8F 8MF models Vacuum Filtration Maintenance Run the predefined link protocol VAC30_TEST with disinfectant as the fluid supply to clean the tubing and har...

Страница 63: ...ay Maintenance Overnight multi day maintenance involves flushing all wash solution out of the instrument and then periodically rinsing and soaking the tubes to keep them moist If the washer will be left idle for a period of time such as overnight or over a weekend run this program to soak the tubes for several hours at a time The OVERNIGHT_LOOP and RINSE_AND_SOAK programs satisfy overnight multi d...

Страница 64: ...DAY_RINSE three times 3 Fill a supply bottle with DI water and connect it to the washer 4 Run DAY_RINSE three times 5 Run OVERNIGHT_LOOP Weekly Using an Enzyme Active Detergent 1 Mix an enzyme active detergent according to the manufacturer s directions to fill a supply bottle Connect the bottle to the washer 2 Run RINSE_AND_SOAK 3 If you plan to use the washer immediately run DAY_RINSE twice with ...

Страница 65: ...ment spray it with liquid or use a wet cloth Do not allow the cleaning solution to run into the interior of the instrument If this happens contact the BioTek Service Department Do not soak the touchscreen this will cause damage Moisten a clean cloth with deionized or distilled water and wipe the touchscreen Dry immediately with a clean dry cloth Do not apply lubricants to manifold gasket manifold ...

Страница 66: ...er and ring out thoroughly Do not use a dripping cloth Avoid fibrous material that can scratch the surface Important If necessary only use the recommended Cleaning solutions and do not use a higher concentration of the solutions If a cleaning solution is used wipe again with a cloth dampened with water only and dry with a clean cloth Clean the Exterior Surfaces 1 Run the system dry connect an empt...

Страница 67: ...Vacuum Filtration Carriers 1 Disconnect the tubing between the vacuum filtration carrier and washer and lift the carrier out of the base if applicable 2 Rinse the carrier under tap water and allow to dry If additional cleaning appears necessary soak the carrier in hot soapy water for 20 minutes Rinse it well and let it dry Inspect the plate seal gasket and replace it if defects are observed Vacuum...

Страница 68: ...nifold Using a lint free disposable towel moistened with water or with water and mild detergent thoroughly clean the outside of the dispense and aspirate tubes Run hot water through both the inlet and outlet fittings Check to see if water comes out of all of the dispense and aspirate tubes If not soak the manifold in hot soapy water and try again Performance problems like uneven aspiration or disp...

Страница 69: ... the Aspirate and Dispense Tubes The manifold dispense tubes have a protective Teflon collar at the tip This is to prevent dripping Do not remove these coverings 8 8s 12 2x8 or 4 Well Manifold 1 Remove the manifold Disconnect the tubing from the back of the manifold Remove the thumbscrews that hold it in place Disassembled 8 channel manifold 2 Use a Phillips screwdriver to remove the metal plate o...

Страница 70: ...Remount the manifold and replace the tubing 8 Replace the mist shield 9 Run a Prime program using 200 mL 10 Verify dispenser performance visually or by completing the Dispense Precision Test 8 8s 2x8 and 12 Manifolds 8 16 Well Manifold Important Do not disassemble the 8 16 Well manifold There are five screws on the top metal plate Removing any of these screws will cause the manifold to fall out of...

Страница 71: ...out the channels by holding the manifold under a running faucet of warm water Alternate between using the stylus and flushing the channel for each tube 4 Rinse the manifold with deionized or distilled water Make sure water comes out of all of the dispense and aspirate tubes 5 When satisfied replace all of the plugs 6 Remount the manifold and replace the tubing 7 Replace the mist shield 8 Run a Pri...

Страница 72: ...bility for the adequacy of these solutions and methods Each laboratory must ensure that decontamination procedures are adequate for the Biohazard s they handle Warning Internal Voltage Turn off and unplug the instrument for all decontamination operations Do not immerse the instrument spray it with liquid or use a wet cloth Do not allow the cleaning solution to run into the interior of the instrume...

Страница 73: ...ormaldehyde 4 glutaraldehyde 1 3 Virkon Deionized or distilled water Safety glasses Surgical mask Protective gloves Lab coat Biohazard trash bags 125 mL beakers Clean cotton cloths or paper towels Decontamination bleach solution disinfectant Prepare an aqueous cleaning solution of 0 5 sodium hypochlorite NaClO As an alternative 70 isopropyl alcohol may be used if the effects of bleach are a concer...

Страница 74: ...s is the case prepare a 1 10 dilution Do not prepare a stronger cleaning solution than described 1 Turn off and unplug the instrument 2 Moisten a cloth with the bleach solution or alcohol Do not soak the cloth 3 Wipe the touchscreen See Clean the Touchscreen on page 12 4 Remove the mist shield if it is attached Wipe the inside and outside mist shield surfaces Wipe the microplate carrier top surfac...

Страница 75: ...aces 1 Connect the washer to the power supply and turn it on 2 Connect the disinfectant fluid supply and empty the waste bottle 3 Run DECONTAMINATION Select Maintenance from the Main Menu While the program is running you will need to periodically check the display and follow instructions for changing the supply bottles 4 Flush all the bleach disinfectant from the instrument by running DAY_RINSE at...

Страница 76: ...id Tests 5 Self Test 10 Dispense Precision Test 8 8s 2X8 and 12 Well 11 Annual Buffer Switching Dispense Precision Test 12 Evacuation Efficiency Test 8 8s 2X8 and 12 Well 13 Vacuum Filtration Evacuation Efficiency Test 14 Dispense Precision Test 8 16 Well 15 Annual Buffer Switching Dispense Precision Test 16 Evacuation Efficiency Test 8 16 Well 17 Dispense Precision Test 4 Well Manifold 18 Evacuat...

Страница 77: ...f the plate after evacuation to verify it is performing to specifications Dispense Precision This test measures the variability of volumes dispensed from tube to tube across the manifold Annual Buffer Switching Test The Dispense Precision Test for the Buffer Switching valves measures the variability of volumes dispensed from tube to tube across the manifold when the washer is equipped with buffer ...

Страница 78: ...uation Efficiency Test Annual Buffer Switching testing can begin after first year of use It is important to note that the risk and performance factors associated with your assays may require that some or all of the procedures be performed more frequently than presented in the schedule Which Tests to Perform We recommend that you perform these routine tests on all manifolds before first use after t...

Страница 79: ...witching V models we recommend performing this additional test annually starting one year after installation Annual Buffer Switching Test Measures the variability of volumes dispensed from tube to tube across the manifold for each valve when the washer is used with this valve switching module If you are testing a biomagnetic separation M model using the magnetic bead carrier do not perform these t...

Страница 80: ...anifold In this test a blue dye solution is dispensed into a microplate The optical density of each well is measured at 630 nm and the background at 450 nm is subtracted to account for scratches on the plate or particulates in the well The average error percentage is calculated and the amount dispensed to each well is calculated Acceptance is based on the CV Coefficient of Variation or the ratio o...

Страница 81: ...fer Switching Test if applicable one plate per valve tested 50 TS 8F and 8MF Models Test 96 well filter plate Millipore MSHVN4550 96 or equivalent 0 45 micron filter plate Vacuum Filtration Evacuation Efficiency Test Precision balance with capacity of 100 g minimum and readability of 0 001 g resolution Pipettes and graduated beakers Microplate absorbance reader capable of dual wavelength reading a...

Страница 82: ...e failure of the washer to meet specifications Prepare the solutions a few hours before running the tests to let the foam created by mixing the Tween solution to settle You may use your own buffer solution in place of Solution 1 However if any tests fail retry the tests using the specified solutions Important BioTek determined the pass fail specifications for the tests using the specified test sol...

Страница 83: ...0 Secondary Aspirate No No Vacuum Filtration models 96 well filter plates Protocol VAC30_TEST Aspiration Type Vacuum Filtration Dispense Height Z Offset 120 Vacuum Filtration Time 30 seconds Dispense Precision Tests accuracy_qc_test accuracy_qc384 Dispense Volume 300 µL well 100 µL well Dispense Flow Rate 5 5 Dispense Height Z Offset 146 120 Y Offset 00 00 Prime Before Start Yes Yes Prime volume a...

Страница 84: ...eps 1 65 mm Y Offset Position 90 4 11 mm Aspirate Travel Rate 1 5 0 mm sec Aspirate Delay 2000 msec Secondary Aspirate No Dispense Precision Test Accuracy QC Test Dispense Volume 1120 µL well Dispense Flow Rate 5 Dispense Height Z Offset 180 steps 22 87 mm Y Offset 00 Prime Before Start Yes Prime volume and flow rate 5 mL at rate 5 ...

Страница 85: ...anually from the Main Menu Select Instrument Options and press Start The Self Test checks the vacuum manifold and manifold to carrier movement If the test passes the Main Menu is displayed and the washer is ready for use If the test fails the washer will beep and display an error code Press OK and look up the code See Error Codes ...

Страница 86: ... a supply bottle and prime the tubing and the manifold Quick Prime System 3 Run the dispense program accuracy_qc_test Enter the number of strips to process 12 for the 8 8s and 2X8Well manifolds or 8 for the 12 Well manifold 4 Read the plate in an absorbance reader blank on air using the dual wavelength method 630 450 nm Print or export the results Note If you are using one of BioTek s keypad based...

Страница 87: ...en as needed throughout this procedure 2 Fill each of the supply bottles connected to Valves A B and C with approximately 200 mL deionized water Repeat the following steps for each valve 3 Run the Maintenance program DAY_RINSE to prime the fluid lines manifold and the valve being tested 4 Place a new 96 well microplate on the balance and zero the balance 5 Place the microplate on the carrier and r...

Страница 88: ...microplate on the balance and tare the balance 3 Pipette or dispense 300 μL of Solution 1 2 or 3 your choice into each well of the microplate 4 Place the plate on the washer and run the Aspirate program residual_qc_test This program evacuates all of the wells leaving a small amount of residual fluid 5 When the program is finished remove the plate and weigh it immediately because evaporation will a...

Страница 89: ...he annual OQ and the monthly PQ 1 Fill a supply bottle at least 200 mL of deionized water Run Maintenance program DAY_RINSE to prime the fluid lines and manifold 2 Place a new microplate on the balance and zero the balance 96 well 0 45 µm filter plate Millipore MSHVN4550 96 is recommended 3 Run the protocol to first dispense DI water into each well of the microplate and then evacuate the wells VAC...

Страница 90: ...ou will be using the same plate for the Evacuation Efficiency Test place a new 384 well plate on the balance and tare the balance 2 Prepare the washer to dispense test Solution 3 to the microplate Test solutions Error Bookmark not defined fill a supply bottle and prime the tubing and the manifold Quick Prime System 3 Run the dispense program accur_qc_test384 Enter the number of strips to process 2...

Страница 91: ...ded throughout this procedure 2 Fill each of the supply bottles connected to Valves A B and C with deionized water Repeat the following steps for each valve 3 Run the Maintenance program DAY_RINSE to prime the fluid lines manifold and the valve being tested 4 Place a new 384 well microplate on the balance and zero the balance 5 Place the microplate on the carrier and run the dispense program accur...

Страница 92: ... Pipette or dispense 80 μL of Solution 1 2 or 3 your choice into each well of the microplate 4 Place the plate on the washer and run the Aspirate program residual_qc_test384 This program evacuates all of the wells leaving a small amount of residual fluid 5 When the program is finished remove the plate and weigh it immediately because evaporation will affect the results This is the Total Residual W...

Страница 93: ...nifold When this test is complete the filled plate can be used to perform the Evacuation Efficiency Test 1 Use a clean dry 24 well microplate If you will be using the same plate for the Evacuation Efficiency Test place a new plate on the balance and tare the balance 2 Prepare the washer to dispense test Solution 3 to the microplate fill a supply bottle and prime the tubing and the manifold Quick P...

Страница 94: ...he program is finished remove the plate and weigh it immediately because evaporation will affect the results This is the Total Residual Weight in grams 6 Visually inspect the plate and note if any wells appear to have considerably more liquid in them than others 7 Using the worksheet at the end of this chapter calculate results o Divide the Total Residual Weight by 24 to find the Mean Residual Wei...

Страница 95: ...otocol at the main menu 2 Select Create 3 Enter a unique name for the new protocol for example 4 Well accuracy_qc_test and 4 Well residual_qc_test 4 Set the Plate Type to 24 and press Save 5 Press Add and select the type of action to perform 6 Define the parameters as shown below and Save the protocols 4 Well Manifold Test Parameters Evacuation Efficiency Test Residual QC Test Aspirate Height Z Of...

Страница 96: ...ents Inc Dispense Precision Test Accuracy QC Test Dispense Volume 1120 µL well Dispense Flow Rate 5 Dispense Height Z Offset 180 steps 22 87 mm Y Offset 00 Prime Before Start Yes Prime volume and flow rate 5 mL at rate 5 ...

Страница 97: ...Section 6 Specifications For your convenience this section contains performance and physical specifications Physical Specifications 2 Performance Specifications 4 ...

Страница 98: ...icrostrips in holders that comply with ANSI SLAS microplate standards 1 2004 2 2004 3 2004 and 4 2004 with a maximum plate height of 800 20 3 mm and flat round or V bottom microwells For example using the 4 Well manifold 24 well Corning 3524 plates are supported 1 The vacuum filtration F and MF models wash 96 well filter bottom plates with filter pore sizes from 0 45 µm to 1 2 µm For example Milli...

Страница 99: ...50 relative humidity at 40 C Muffler A silencing muffler PN 4073009 to reduce vacuum pump noise is provided Performance criteria shall be met with the muffler installed Bottles Waste bottle volume 2 liters 4L optional Supply bottle2 volume 2 liters 4L optional Liquid level detection is available as an optional accessory Plate Carrier Carrier Manifold Types Instrument Model Standard All manifold ty...

Страница 100: ...ll 3 0 CV when measured over four 300 µL per well dispenses of deionized water with 0 1 Tween 20 8 16 Well 384 well 4 0 CV when measured over six 100 µL per well dispenses of deionized water with 0 1 Tween 20 2x8 Well 96 well 4 0 CV when measured over six 300 µL per well dispenses of deionized water with 0 1 Tween 20 4 Well 24 well 4 0 CV when measured over six 1120 µL per well dispenses of deioni...

Страница 101: ...uching well aspiration and a solution of deionized water with 0 1 Tween 20 or equivalent buffer solution The aspirate height should be optimized for the plate prior to testing 2x8 Well 96 well Average residual of 4 0 µL per well after a 3 cycle wash when dispensing 300 µL per well using a bottom touching well aspiration and a solution of deionized water with 0 1 Tween 20 or equivalent buffer solut...

Страница 102: ... the first strip and is stopped when the last strip has been aspirated Manifold Type Plate Type Performance 8 and 8s Well 96 well 130 seconds for 12 strips 3 cycles 300 µL well no soak 8 16 Well 384 well 260 seconds for 24 strips 3 cycles 100 µL well no soak 2x8 Well 96 well 80 seconds for 12 strips 3 cycles 300 µl well no soak 4 Well 24 well 60 seconds for a 24 well plate 1 cycle 1120 µl well no ...

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