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BioTek Instruments, Inc.
Aspiration Protocol Parameters
Options
Actions
Default
Aspirate Type
Standard aspiration using the manifold or Vacuum
Filtration for 8F and 8MF models using the vacuum
filtration carrier.
Filtration Time
When Aspirate Type is set to Vacuum Filtration. The
range is 1-180 seconds. Experimentation is
recommended to determine the optimal setting for your
assays.
30
Aspirate Height
(Z-axis)
The height between the bottom of the aspirate tubes and
the carrier surface when aspirating the microwells. The
aspirate tubes are longer than the dispense tubes.
Ranges from 12 to 180 steps. The washer shows the
corresponding millimeters. 12 is the closest to the carrier,
180 is the furthest.
24
Horizontal Position
(Y-axis)
The position of the manifold tubes in relation to the
center of the microwells.
The Y-axis range is from -24 to 24 for the 8- and 12-
channel manifolds and the 2x8 manifold; -88 to 24 for
the 8s-manifold; -24 to 15 for the 16-channel when
addressing 96-well plates and -22 to 10 for 384-well
plates; the 4 (24-well plate) manifold range is -180-60.
The washer displays the corresponding millimeters.
A negative offset moves the aspirate tubes back, away
from the front of the washer. A positive offset moves the
tubes toward the front of the washer. A setting of 0
indicates no offset.
Note: For flat-bottom plates, position the aspirate tubes
near the edge of the well for minimal residual volume.
0
Travel Rate
The rate at which the wash manifold travels down into
the wells. Options range from 1 to 7, where 1 is slowest
and 7 is fastest. The washer shows the corresponding
mm/sec value for each option.
3
Aspirate Delay
The time delay starting when the tube is at its aspiration
height and ending when it next moves. The valid range is
0 to 5000 milliseconds.
The delay applies to the normal (initial) aspiration when
Secondary (aka Crosswise) Aspirate is disabled. If
Secondary Aspiration is enabled, the delay applies to the
second, not the initial, aspiration.
0
