18 |
50™ TS Washer
•
Failure may occur when a microplate other than the recommended Corning
®
Costar 384 is used. If you prefer to use a different plate, create a new
protocol based on the original
residual_qc_test384
but change the
Aspirate height (Z Offset) or other parameters to accommodate the
different plate geometry. Then, retry the test using a new microplate. (See
Liquid Test Protocols: Default Parameters on page 7.)
•
Review possible causes of poor aspiration in the Troubleshooting section in
the operator's manual.
If the test fails a second time: contact BioTek TAC for assistance.
Dispense Precision Test (4 Well Manifold)
Important: You must create your own qualification test protocols for this
manifold type:
See Create Qualification Protocols for 4 Well Manifold on
page 20.
Make sure the Instrument>Manifold setting matches the installed 4 Well
manifold.
When this test is complete, the filled plate can be used to perform the
Evacuation Efficiency Test.
1.
Use a clean, dry 24-well microplate. If you will be using the same plate for
the
Evacuation Efficiency Test
: place a new plate on the balance and tare
the balance.
2.
Prepare the washer to dispense test Solution #3 to the microplate: fill a
supply bottle and prime the tubing and the manifold
(
Quick>Prime>System
).
3.
Run the protocol created for the test, for example
4-Well
accuracy_qc_test
. Enter
6
for the number of strips to process.
4.
Read the plate in an absorbance reader (blank on air) using the dual-
wavelength method (630-450 nm). Print or export the results.
5.
Calculate and report the mean absorbance, standard deviation, and %
Coefficient of Variation (%CV) for the plate.
A %CV greater than 4.0% is a failure. If your result is greater than 4.0%,
clean the dispense tubes with a stylus and retest.
If this test does not pass on the initial attempt, use the stylus to clean the
dispense tubes that have OD reading results lower than average, and then
retest.
