Waters nanoACQUITY UPLC, Quick Start Manual

Page: 65 / 76

4-1
4
Verifying System Operation
This chapter explains how to run a gradient performance test to verify that
the system is operating properly. The sample you use to verify the system is
included in the system startup kit.
Before you begin this procedure, the system must be set up and configured as
described in the nanoACQUITY UPLC System Operator’s Guide.
Required materials
Make sure these materials are on hand before you begin the verification test:
• MS-grade water
• acetonitrile (Waters recommends Fisher Scientific’s Optima
®
brand)
• MS-grade formic acid
• MassPREP™ Peptide Standard (system startup kit)
• nanoACQUITY BEH C18 analytical column (1.7
µ
m, 100
µ
m
×
100 mm)
• nanoACQUITY Symmetry C18 trap column (5
µ
m, 180
µ
m
×
20 mm)
• powder-free nitrile gloves
Contents:
Topic Page
Required materials 4-1
Preparing the mobile phases 4-2
Preparing the sample 4-3
Preparing the system 4-4
Creating the test methods 4-6
Performing the test 4-10